Determination of protein phosphorylation sites by mass spectrometry: A novel electrospray-based method

Vera Alverdi, Francesca Di Pancrazio, Giovanna Lippe, Carlo Pucillo, Bruno Casetta, Gennaro Esposito

Research output: Contribution to journalArticle

Abstract

Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO 3-deprived form using the capabilities of tandem mass spectrometers.

Original languageEnglish (US)
Pages (from-to)3343-3348
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume19
Issue number22
DOIs
StatePublished - Dec 2 2005

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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