Determination of protein phosphorylation sites by mass spectrometry: A novel electrospray-based method

Vera Alverdi, Francesca Di Pancrazio, Giovanna Lippe, Carlo Pucillo, Bruno Casetta, Gennaro Esposito

Research output: Contribution to journalArticle

Abstract

Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO 3-deprived form using the capabilities of tandem mass spectrometers.

Original languageEnglish (US)
Pages (from-to)3343-3348
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume19
Issue number22
DOIs
StatePublished - Dec 2 2005

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Phosphorylation
Mass spectrometry
Liquid chromatography
Mass spectrometers
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Cite this

Determination of protein phosphorylation sites by mass spectrometry : A novel electrospray-based method. / Alverdi, Vera; Di Pancrazio, Francesca; Lippe, Giovanna; Pucillo, Carlo; Casetta, Bruno; Esposito, Gennaro.

In: Rapid Communications in Mass Spectrometry, Vol. 19, No. 22, 02.12.2005, p. 3343-3348.

Research output: Contribution to journalArticle

Alverdi, Vera ; Di Pancrazio, Francesca ; Lippe, Giovanna ; Pucillo, Carlo ; Casetta, Bruno ; Esposito, Gennaro. / Determination of protein phosphorylation sites by mass spectrometry : A novel electrospray-based method. In: Rapid Communications in Mass Spectrometry. 2005 ; Vol. 19, No. 22. pp. 3343-3348.
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