Determination of effective synaptic conductances using somatic voltage clamp

Songting Li, Nan Liu, Li Yao, Xiaohui Zhang, Doug Zhou, David Cai

Research output: Contribution to journalArticle

Abstract

The interplay between excitatory and inhibitory neurons imparts rich functions of the brain. To understand the synaptic mechanisms underlying neuronal computations, a fundamental approach is to study the dynamics of excitatory and inhibitory synaptic inputs of each neuron. The traditional method of determining input conductance, which has been applied for decades, employs the synaptic current-voltage (I-V) relation obtained via voltage clamp. Due to the space clamp effect, the measured conductance is different from the local conductance on the dendrites. Therefore, the interpretation of the measured conductance remains to be clarified. Using theoretical analysis, electrophysiological experiments, and realistic neuron simulations, here we demonstrate that there does not exist a transform between the local conductance and the conductance measured by the traditional method, due to the neglect of a nonlinear interaction between the clamp current and the synaptic current in the traditional method. Consequently, the conductance determined by the traditional method may not correlate with the local conductance on the dendrites, and its value could be unphysically negative as observed in experiment. To circumvent the challenge of the space clamp effect and elucidate synaptic impact on neuronal information processing, we propose the concept of effective conductance which is proportional to the local conductance on the dendrite and reflects directly the functional influence of synaptic inputs on somatic membrane potential dynamics, and we further develop a framework to determine the effective conductance accurately. Our work suggests re-examination of previous studies involving conductance measurement and provides a reliable approach to assess synaptic influence on neuronal computation.

Original languageEnglish (US)
Pages (from-to)e1006871
JournalPLoS computational biology
Volume15
Issue number3
DOIs
StatePublished - Mar 1 2019

Fingerprint

Clamping devices
Conductance
dendrites
Dendrites
Voltage
Neurons
neurons
Electric potential
information processing
Dendrite
methodology
Automatic Data Processing
membrane potential
Membrane Potentials
brain
Neuron
Brain
transform
experiment
Experiments

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Determination of effective synaptic conductances using somatic voltage clamp. / Li, Songting; Liu, Nan; Yao, Li; Zhang, Xiaohui; Zhou, Doug; Cai, David.

In: PLoS computational biology, Vol. 15, No. 3, 01.03.2019, p. e1006871.

Research output: Contribution to journalArticle

Li, Songting ; Liu, Nan ; Yao, Li ; Zhang, Xiaohui ; Zhou, Doug ; Cai, David. / Determination of effective synaptic conductances using somatic voltage clamp. In: PLoS computational biology. 2019 ; Vol. 15, No. 3. pp. e1006871.
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