Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.

Karen Yao, Nahid Akyani, Donatella Donati, Naomi Sengamalay, Julie Fotheringham, Elodie Ghedin, Michael Bishop, John Barrett, Fatah Kashanchi, Steven Jacobson

Research output: Contribution to journalArticle

Abstract

BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.

Fingerprint

Human Herpesvirus 6
Nerve Tissue
Central Nervous System
Bone Marrow
Viruses
Viral RNA
Viral Load
Transplants
Viral DNA
Brain Diseases
Infection
Nervous System Diseases
Cerebral Cortex
Nervous System
Transplant Recipients
Real-Time Polymerase Chain Reaction
Autopsy
Polymerase Chain Reaction
Incidence
Brain

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient : a multi-virus array analysis. / Yao, Karen; Akyani, Nahid; Donati, Donatella; Sengamalay, Naomi; Fotheringham, Julie; Ghedin, Elodie; Bishop, Michael; Barrett, John; Kashanchi, Fatah; Jacobson, Steven.

In: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, Vol. 37 Suppl 1, 12.2006.

Research output: Contribution to journalArticle

Yao, Karen ; Akyani, Nahid ; Donati, Donatella ; Sengamalay, Naomi ; Fotheringham, Julie ; Ghedin, Elodie ; Bishop, Michael ; Barrett, John ; Kashanchi, Fatah ; Jacobson, Steven. / Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient : a multi-virus array analysis. In: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 2006 ; Vol. 37 Suppl 1.
@article{060f22f59b4141229ac4b293fbab410b,
title = "Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient: a multi-virus array analysis.",
abstract = "BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46{\%}) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.",
author = "Karen Yao and Nahid Akyani and Donatella Donati and Naomi Sengamalay and Julie Fotheringham and Elodie Ghedin and Michael Bishop and John Barrett and Fatah Kashanchi and Steven Jacobson",
year = "2006",
month = "12",
doi = "10.1016/S1386-6532(06)70013-0",
language = "English (US)",
volume = "37 Suppl 1",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",

}

TY - JOUR

T1 - Detection of HHV-6B in post-mortem central nervous system tissue of a post-bone marrow transplant recipient

T2 - a multi-virus array analysis.

AU - Yao, Karen

AU - Akyani, Nahid

AU - Donati, Donatella

AU - Sengamalay, Naomi

AU - Fotheringham, Julie

AU - Ghedin, Elodie

AU - Bishop, Michael

AU - Barrett, John

AU - Kashanchi, Fatah

AU - Jacobson, Steven

PY - 2006/12

Y1 - 2006/12

N2 - BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.

AB - BACKGROUND: HHV-6 has been implicated in a number of neurological disorders. Recent evidence has suggested high incidence of HHV-6 infection in patients (46%) undergoing allogeneic bone marrow transplant (BMT). OBJECTIVE: To investigate whether HHV-6 plays a role in the development of fatal encephalopathy in an allogeneic post-BMT patient using an unbiased approach. RESULTS: Detection of HHV-6 viral DNA sequence and RNA expression were demonstrated in fresh frozen post-mortem autopsy material derived from the insular cortex using a multi-virus array platform. In addition, PCR analysis by real-time quantitative TaqMan demonstrated high viral burden in multiple brain regions tested. Sequencing analysis of PCR product confirmed the virus to be HHV-6 variant B. CONCLUSIONS: Active infection as demonstrated by expression of viral RNA and high viral load in the CNS suggest a possible pathogenic role of HHV-6 in development neurologic complications post-BMT.

UR - http://www.scopus.com/inward/record.url?scp=34548047667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548047667&partnerID=8YFLogxK

U2 - 10.1016/S1386-6532(06)70013-0

DO - 10.1016/S1386-6532(06)70013-0

M3 - Article

C2 - 17276371

AN - SCOPUS:34548047667

VL - 37 Suppl 1

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

ER -