Detecting single stranded DNA with a solid state nanopore

Daniel Fologea, Marc Gershow, Bradley Ledden, David S. McNabb, Jene A. Golovchenko, Jiali Li

    Research output: Contribution to journalArticle

    Abstract

    Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.

    Original languageEnglish (US)
    Pages (from-to)1905-1909
    Number of pages5
    JournalNano Letters
    Volume5
    Issue number10
    DOIs
    StatePublished - Oct 2005

    Fingerprint

    Nanopores
    Single-Stranded DNA
    DNA
    deoxyribonucleic acid
    solid state
    Molecules
    molecules
    sequencing
    micrometers
    detectors
    electric potential
    configurations
    Detectors
    Electric potential

    ASJC Scopus subject areas

    • Materials Science(all)
    • Electronic, Optical and Magnetic Materials
    • Chemistry (miscellaneous)

    Cite this

    Fologea, D., Gershow, M., Ledden, B., McNabb, D. S., Golovchenko, J. A., & Li, J. (2005). Detecting single stranded DNA with a solid state nanopore. Nano Letters, 5(10), 1905-1909. https://doi.org/10.1021/nl051199m

    Detecting single stranded DNA with a solid state nanopore. / Fologea, Daniel; Gershow, Marc; Ledden, Bradley; McNabb, David S.; Golovchenko, Jene A.; Li, Jiali.

    In: Nano Letters, Vol. 5, No. 10, 10.2005, p. 1905-1909.

    Research output: Contribution to journalArticle

    Fologea, D, Gershow, M, Ledden, B, McNabb, DS, Golovchenko, JA & Li, J 2005, 'Detecting single stranded DNA with a solid state nanopore', Nano Letters, vol. 5, no. 10, pp. 1905-1909. https://doi.org/10.1021/nl051199m
    Fologea D, Gershow M, Ledden B, McNabb DS, Golovchenko JA, Li J. Detecting single stranded DNA with a solid state nanopore. Nano Letters. 2005 Oct;5(10):1905-1909. https://doi.org/10.1021/nl051199m
    Fologea, Daniel ; Gershow, Marc ; Ledden, Bradley ; McNabb, David S. ; Golovchenko, Jene A. ; Li, Jiali. / Detecting single stranded DNA with a solid state nanopore. In: Nano Letters. 2005 ; Vol. 5, No. 10. pp. 1905-1909.
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