Design of immobile nucleic acid junctions.

Nadrian Seeman, N. R. Kallenbach

Research output: Contribution to journalArticle

Abstract

Nucleic acids that interact to generate structures in which three or more double helices emanate from a single point are said to form a junction. Such structures arise naturally as intermediates in DNA replication and recombination. It has been proposed that stable junctions can be created by synthesizing sets of oligonucleotides of defined sequence that can associate by maximizing Watson-Crick complementarity (Seeman N. C., 1981, Biomolecular Stereodynamics. Adenine Press, New York. 1: 269-278; Seeman, N. C., 1982, J. Theor. Biol. 99:237-247.) To make it possible to design molecules that will form junctions of specific architecture, we present here an efficient algorithm for generating nucleic acid sequences that optimize two fundamental properties: fidelity and stability. Fidelity refers to the relative probability of forming the junction complex relative to all alternative paired structures. Calculations are described that permit approximate prediction of the melting curves for junction complexes.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalBiophysical Journal
Volume44
Issue number2
StatePublished - Nov 1983

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Nucleic Acids
Adenine
DNA Replication
Oligonucleotides
Freezing
Genetic Recombination

ASJC Scopus subject areas

  • Biophysics

Cite this

Seeman, N., & Kallenbach, N. R. (1983). Design of immobile nucleic acid junctions. Biophysical Journal, 44(2), 201-209.

Design of immobile nucleic acid junctions. / Seeman, Nadrian; Kallenbach, N. R.

In: Biophysical Journal, Vol. 44, No. 2, 11.1983, p. 201-209.

Research output: Contribution to journalArticle

Seeman, N & Kallenbach, NR 1983, 'Design of immobile nucleic acid junctions.', Biophysical Journal, vol. 44, no. 2, pp. 201-209.
Seeman, Nadrian ; Kallenbach, N. R. / Design of immobile nucleic acid junctions. In: Biophysical Journal. 1983 ; Vol. 44, No. 2. pp. 201-209.
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