Design and construction of a uniaxial cell stretcher

Michael J. Yost, David Simpson, Kimberly Wrona, Stephen Ridley, Harry J. Ploehn, Thomas K. Borg, Louis Terracio

Research output: Contribution to journalArticle

Abstract

In vitro mechanical cell stimulators are used for the study of the effect of mechanical stimulation on anchorage-dependent cells. We developed a new mechanical cell stimulator, which uses stepper motor technology and computer control to achieve a high degree of accuracy and repeatability. This device also uses high-performance plastic components that have been shown to be noncytotoxic, dimensionally stable, and resistant to chemical degradation from common culture laboratory chemicals. We show that treatment with glow discharge for 25 s at 20 mA is sufficient to modify the surface of the rubber to allow proper adhesion for polymerization of aligned collagen. We show through finite element analysis that the middle area of the membrane, away from the clamped ends, is predictable, homogeneous, and has negligible shear strain. To test the efficacy of the mechanical stretch, we examined the effect of mechanical stimulation on the production of β1-integrin by neonatal rat cardiac fibroblasts. Mechanical stimulation was tested in the range of 0-12% stretch and 0-10-cycles/min stretch frequency. The fibroblasts respond with an increase in β1 integrin at 3% stretch and a decrease at 6 and 12% stretch. Stretch frequency was found to not significantly effect the concentration of β1-integrin. These studies yield a new and improved mechanical cell stimulator and demonstrate that mechanical stimulation has an effect on the expression of β1-integrin.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume279
Issue number6 48-6
StatePublished - 2000

Fingerprint

Stretchers
Integrins
Fibroblasts
Laboratory Chemicals
Finite Element Analysis
Rubber
Polymerization
Plastics
Collagen
Technology
Equipment and Supplies
Membranes

Keywords

  • Fibroblasts
  • Integrins
  • Mechanical cell stimulator

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Yost, M. J., Simpson, D., Wrona, K., Ridley, S., Ploehn, H. J., Borg, T. K., & Terracio, L. (2000). Design and construction of a uniaxial cell stretcher. American Journal of Physiology - Heart and Circulatory Physiology, 279(6 48-6).

Design and construction of a uniaxial cell stretcher. / Yost, Michael J.; Simpson, David; Wrona, Kimberly; Ridley, Stephen; Ploehn, Harry J.; Borg, Thomas K.; Terracio, Louis.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 279, No. 6 48-6, 2000.

Research output: Contribution to journalArticle

Yost, MJ, Simpson, D, Wrona, K, Ridley, S, Ploehn, HJ, Borg, TK & Terracio, L 2000, 'Design and construction of a uniaxial cell stretcher', American Journal of Physiology - Heart and Circulatory Physiology, vol. 279, no. 6 48-6.
Yost MJ, Simpson D, Wrona K, Ridley S, Ploehn HJ, Borg TK et al. Design and construction of a uniaxial cell stretcher. American Journal of Physiology - Heart and Circulatory Physiology. 2000;279(6 48-6).
Yost, Michael J. ; Simpson, David ; Wrona, Kimberly ; Ridley, Stephen ; Ploehn, Harry J. ; Borg, Thomas K. ; Terracio, Louis. / Design and construction of a uniaxial cell stretcher. In: American Journal of Physiology - Heart and Circulatory Physiology. 2000 ; Vol. 279, No. 6 48-6.
@article{515ca22ab47b443aa2bb899c0e75eb74,
title = "Design and construction of a uniaxial cell stretcher",
abstract = "In vitro mechanical cell stimulators are used for the study of the effect of mechanical stimulation on anchorage-dependent cells. We developed a new mechanical cell stimulator, which uses stepper motor technology and computer control to achieve a high degree of accuracy and repeatability. This device also uses high-performance plastic components that have been shown to be noncytotoxic, dimensionally stable, and resistant to chemical degradation from common culture laboratory chemicals. We show that treatment with glow discharge for 25 s at 20 mA is sufficient to modify the surface of the rubber to allow proper adhesion for polymerization of aligned collagen. We show through finite element analysis that the middle area of the membrane, away from the clamped ends, is predictable, homogeneous, and has negligible shear strain. To test the efficacy of the mechanical stretch, we examined the effect of mechanical stimulation on the production of β1-integrin by neonatal rat cardiac fibroblasts. Mechanical stimulation was tested in the range of 0-12{\%} stretch and 0-10-cycles/min stretch frequency. The fibroblasts respond with an increase in β1 integrin at 3{\%} stretch and a decrease at 6 and 12{\%} stretch. Stretch frequency was found to not significantly effect the concentration of β1-integrin. These studies yield a new and improved mechanical cell stimulator and demonstrate that mechanical stimulation has an effect on the expression of β1-integrin.",
keywords = "Fibroblasts, Integrins, Mechanical cell stimulator",
author = "Yost, {Michael J.} and David Simpson and Kimberly Wrona and Stephen Ridley and Ploehn, {Harry J.} and Borg, {Thomas K.} and Louis Terracio",
year = "2000",
language = "English (US)",
volume = "279",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "6 48-6",

}

TY - JOUR

T1 - Design and construction of a uniaxial cell stretcher

AU - Yost, Michael J.

AU - Simpson, David

AU - Wrona, Kimberly

AU - Ridley, Stephen

AU - Ploehn, Harry J.

AU - Borg, Thomas K.

AU - Terracio, Louis

PY - 2000

Y1 - 2000

N2 - In vitro mechanical cell stimulators are used for the study of the effect of mechanical stimulation on anchorage-dependent cells. We developed a new mechanical cell stimulator, which uses stepper motor technology and computer control to achieve a high degree of accuracy and repeatability. This device also uses high-performance plastic components that have been shown to be noncytotoxic, dimensionally stable, and resistant to chemical degradation from common culture laboratory chemicals. We show that treatment with glow discharge for 25 s at 20 mA is sufficient to modify the surface of the rubber to allow proper adhesion for polymerization of aligned collagen. We show through finite element analysis that the middle area of the membrane, away from the clamped ends, is predictable, homogeneous, and has negligible shear strain. To test the efficacy of the mechanical stretch, we examined the effect of mechanical stimulation on the production of β1-integrin by neonatal rat cardiac fibroblasts. Mechanical stimulation was tested in the range of 0-12% stretch and 0-10-cycles/min stretch frequency. The fibroblasts respond with an increase in β1 integrin at 3% stretch and a decrease at 6 and 12% stretch. Stretch frequency was found to not significantly effect the concentration of β1-integrin. These studies yield a new and improved mechanical cell stimulator and demonstrate that mechanical stimulation has an effect on the expression of β1-integrin.

AB - In vitro mechanical cell stimulators are used for the study of the effect of mechanical stimulation on anchorage-dependent cells. We developed a new mechanical cell stimulator, which uses stepper motor technology and computer control to achieve a high degree of accuracy and repeatability. This device also uses high-performance plastic components that have been shown to be noncytotoxic, dimensionally stable, and resistant to chemical degradation from common culture laboratory chemicals. We show that treatment with glow discharge for 25 s at 20 mA is sufficient to modify the surface of the rubber to allow proper adhesion for polymerization of aligned collagen. We show through finite element analysis that the middle area of the membrane, away from the clamped ends, is predictable, homogeneous, and has negligible shear strain. To test the efficacy of the mechanical stretch, we examined the effect of mechanical stimulation on the production of β1-integrin by neonatal rat cardiac fibroblasts. Mechanical stimulation was tested in the range of 0-12% stretch and 0-10-cycles/min stretch frequency. The fibroblasts respond with an increase in β1 integrin at 3% stretch and a decrease at 6 and 12% stretch. Stretch frequency was found to not significantly effect the concentration of β1-integrin. These studies yield a new and improved mechanical cell stimulator and demonstrate that mechanical stimulation has an effect on the expression of β1-integrin.

KW - Fibroblasts

KW - Integrins

KW - Mechanical cell stimulator

UR - http://www.scopus.com/inward/record.url?scp=0034532651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034532651&partnerID=8YFLogxK

M3 - Article

VL - 279

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 6 48-6

ER -