Design and characterization of fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein

Priya Katyal, Jin Montclare

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tremendous effort has been dedicated to the design and assembly of bioinspired protein-based architectures with potential applications in drug delivery, tissue engineering, biosensing, and bioimaging. Here, we describe our strategy to generate fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein (COMPcc). Our construct, Q, engineered by swapping particular regions of COMPcc to optimize surface charge, self-assembles to form nanofibers. The Q protein nanofibers can efficiently bind curcumin to form robust mesofibers that can be potentially used for drug delivery and biomedical applications. In addition, using the same Q protein, we describe the biotemplation of gold nanoparticles (AuNP) in the presence and absence of the hexahistidine tag (His-tag). The Q bearing His-tag·AuNP (Q·AuNP) readily deposits on electrode surfaces, while Q without His-tag·AuNP (Qx·AuNP) stabilizes the soluble protein·gold bionanocomposites for several days without aggregating.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages239-263
Number of pages25
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1798
ISSN (Print)1064-3745

Fingerprint

Cartilage Oligomeric Matrix Protein
Nanofibers
His-His-His-His-His-His
Proteins
Curcumin
Tissue Engineering
Gold
Pharmaceutical Preparations
Nanoparticles
Electrodes

Keywords

  • Bionanocomposites
  • Cartilage oligomeric matrix protein
  • Coiled-coil protein
  • Domain-swapped
  • Fibers
  • Gold nanoparticles
  • Self-assembly

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Katyal, P., & Montclare, J. (2018). Design and characterization of fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein. In Methods in Molecular Biology (pp. 239-263). (Methods in Molecular Biology; Vol. 1798). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7893-9_19

Design and characterization of fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein. / Katyal, Priya; Montclare, Jin.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 239-263 (Methods in Molecular Biology; Vol. 1798).

Research output: Chapter in Book/Report/Conference proceedingChapter

Katyal, P & Montclare, J 2018, Design and characterization of fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1798, Humana Press Inc., pp. 239-263. https://doi.org/10.1007/978-1-4939-7893-9_19
Katyal, Priya ; Montclare, Jin. / Design and characterization of fibers and bionanocomposites using the coiled-coil domain of cartilage oligomeric matrix protein. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 239-263 (Methods in Molecular Biology).
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