Dental imaging using mesoscopic fluorescence molecular tomography: An ex vivo feasibility study

Feixiao Long, Mehmet S. Ozturk, Mark Wolff, Xavier Intes, Shiva P. Kotha

Research output: Contribution to journalArticle

Abstract

Some dental lesions are difficult to detect with traditional anatomical imaging methods, such as, with visual observation, dental radiography and X-ray computed tomography (CT). Therefore, we investigated the viability of using an optical imaging technique, Mesoscopic Fluorescence Molecular Tomography (MFMT) to retrieve molecular contrast in dental samples. To establish feasibility of obtaining 3-D images in teeth using MFMT, molecular contrast was simulated using a dye-filled capillary that was placed in the lower half of human tooth ex vivo. The dye and excitation wavelength were chosen to be excited at 650-660 nm in order to simulate a carious lesion. The location of the capillary was varied by changing the depth from the surface at which the dye, at various concentrations, was introduced. MFMT reconstructions were benchmarked against micro-CT. Overall; MFMT exhibited a location accuracy of ~15% and a volume accuracy of ~15%, up to 2 mm depth with moderate dye concentrations. These results demonstrate the potential of MFMT to retrieve molecular contrast in 3-D in highly scattering tissues, such as teeth.

Original languageEnglish (US)
Pages (from-to)488-502
Number of pages15
JournalPhotonics
Volume1
Issue number4
DOIs
StatePublished - Dec 1 2014

Fingerprint

Feasibility Studies
Tomography
Tooth
tomography
Fluorescence
Imaging techniques
fluorescence
Coloring Agents
teeth
Dyes
dyes
lesions
Dental Radiography
X Ray Computed Tomography
Three-Dimensional Imaging
Optical Imaging
visual observation
Radiography
radiography
viability

Keywords

  • Dental imaging
  • Laminar optical tomography
  • Mesoscopic molecular fluorescence tomography
  • Monte Carlo

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Dental imaging using mesoscopic fluorescence molecular tomography : An ex vivo feasibility study. / Long, Feixiao; Ozturk, Mehmet S.; Wolff, Mark; Intes, Xavier; Kotha, Shiva P.

In: Photonics, Vol. 1, No. 4, 01.12.2014, p. 488-502.

Research output: Contribution to journalArticle

Long, Feixiao ; Ozturk, Mehmet S. ; Wolff, Mark ; Intes, Xavier ; Kotha, Shiva P. / Dental imaging using mesoscopic fluorescence molecular tomography : An ex vivo feasibility study. In: Photonics. 2014 ; Vol. 1, No. 4. pp. 488-502.
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