Dendrimers functionalized with membrane-interacting peptides for viral inhibition

Rossella Tarallo, Tom P. Carberry, Annarita Falanga, Mariateresa Vitiello, Stefania Galdiero, Massimiliano Galdiero, Marcus Weck

Research output: Contribution to journalArticle

Abstract

This contribution reports the synthesis of a poly(amide)-based dendrimer functionalized at the termini with a membrane-interacting peptide derived from the herpes simplex virus (HSV) type 1 glycoprotein H, namely gH625-644. This peptide has been shown to interact with model membranes and to inhibit viral infectivity. The peptidodendrimer inhibits both HSV-1 and HSV-2 at a very early stage of the entry process, most likely through an interaction with the viral envelope glycoproteins; thus, preventing the virus from coming into close contact with cellular membranes, a prerequisite of viral internalization. The 50% inhibitory concentration was 100 and 300 nM against HSV-1 and HSV-2 respectively, with no evidence of cell toxicity at these concentrations. These results show that the functionalization of a dendrimer with the peptide sequence derived from an HSV glycoprotein shows promising inhibitory activity towards viruses of the Herpesviridae family.

Original languageEnglish (US)
Pages (from-to)521-534
Number of pages14
JournalInternational Journal of Nanomedicine
Volume8
DOIs
StatePublished - Feb 2 2013

Fingerprint

Dendrimers
Human Herpesvirus 1
Viruses
Peptides
Human Herpesvirus 2
Membranes
Glycoproteins
Herpesviridae
Simplexvirus
Amides
Inhibitory Concentration 50
Toxicity

Keywords

  • Antiviral activity
  • Membranotropic peptides
  • Peptidodendrimer

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Dendrimers functionalized with membrane-interacting peptides for viral inhibition. / Tarallo, Rossella; Carberry, Tom P.; Falanga, Annarita; Vitiello, Mariateresa; Galdiero, Stefania; Galdiero, Massimiliano; Weck, Marcus.

In: International Journal of Nanomedicine, Vol. 8, 02.02.2013, p. 521-534.

Research output: Contribution to journalArticle

Tarallo, R, Carberry, TP, Falanga, A, Vitiello, M, Galdiero, S, Galdiero, M & Weck, M 2013, 'Dendrimers functionalized with membrane-interacting peptides for viral inhibition', International Journal of Nanomedicine, vol. 8, pp. 521-534. https://doi.org/10.2147/IJN.S37739
Tarallo R, Carberry TP, Falanga A, Vitiello M, Galdiero S, Galdiero M et al. Dendrimers functionalized with membrane-interacting peptides for viral inhibition. International Journal of Nanomedicine. 2013 Feb 2;8:521-534. https://doi.org/10.2147/IJN.S37739
Tarallo, Rossella ; Carberry, Tom P. ; Falanga, Annarita ; Vitiello, Mariateresa ; Galdiero, Stefania ; Galdiero, Massimiliano ; Weck, Marcus. / Dendrimers functionalized with membrane-interacting peptides for viral inhibition. In: International Journal of Nanomedicine. 2013 ; Vol. 8. pp. 521-534.
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