De novo mRNA synthesis is required for both consolidation and reconsolidation of fear memories in the amygdala

Sevil Duvarci, Karim Nader, Joseph Ledoux

Research output: Contribution to journalArticle

Abstract

Memory consolidation is the process by which newly learned information is stabilized into long-term memory (LTM). Considerable evidence indicates that retrieval of a consolidated memory returns it to a labile state that requires it to be restabilized. Consolidation of new fear memories has been shown to require de novo RNA and protein synthesis in the lateral nucleus of the amygdala (LA). We have previously shown that de novo protein synthesis in the LA is required for reconsolidation of auditory fear memories. One key question is whether protein synthesis during reconsolidation depends on already existing mRNAs or on synthesis of new mRNAs in the amygdala. In the present study, we examined the effect of mRNA synthesis inhibition during consolidation and reconsolidation of auditory fear memories. We first show that intra-LA infusion of two different mRNA inhibitors dose-dependently impairs long-term memory but leaves short-term memory (STM) intact. Next, we show that intra-LA infusion of the same inhibitors dose-dependently blocks post-reactivation long-term memory (PR-LTM), whereas post-reactivation short-term memory (PR-STM) is left intact. Furthermore, the same treatment in the absence of memory reactivation has no effect. Together, these results show that both consolidation and reconsolidation of auditory fear memories require de novo mRNA synthesis and are equally sensitive to disruption of de novo mRNA synthesis in the LA.

Original languageEnglish (US)
Pages (from-to)747-755
Number of pages9
JournalLearning and Memory
Volume15
Issue number10
DOIs
StatePublished - Oct 2008

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Amygdala
Fear
Messenger RNA
Long-Term Memory
Short-Term Memory
Proteins
RNA

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience
  • Neuropsychology and Physiological Psychology

Cite this

De novo mRNA synthesis is required for both consolidation and reconsolidation of fear memories in the amygdala. / Duvarci, Sevil; Nader, Karim; Ledoux, Joseph.

In: Learning and Memory, Vol. 15, No. 10, 10.2008, p. 747-755.

Research output: Contribution to journalArticle

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