D1 receptor modulation of action potential firing in a subpopulation of layer 5 pyramidal neurons in the prefrontal cortex

Hannah J. Seong, Adam Carter

Research output: Contribution to journalArticle

Abstract

Dopamine modulation in the prefrontal cortexis important for cognitive processing and disrupted in diverse neuropsychiatric diseases. Activation of D1 receptorsis thought to enable working memory by enhancing the firing properties of pyramidalneurons. However, these receptors are only sparsely expressed in the prefrontal cortex, and how they impact individual neurons remains unknown. Here we study D1 receptor modulation of layer 5 pyramidal neurons in acute slices of the mouse prefrontal cortex. Using whole-cell recordings and two-photon microscopy, we show that neurons expressing D1 receptors have unique morphological and physiological properties. We then demonstrate that activation of these receptors selectively enhances the firing of these neurons by signaling via the protein kinase A pathway. This finding of robust D1 receptor modulation in only a subpopulation of neurons has important implications for cognitive function and disease.

Original languageEnglish (US)
Pages (from-to)10516-10521
Number of pages6
JournalJournal of Neuroscience
Volume32
Issue number31
DOIs
StatePublished - Aug 1 2012

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Pyramidal Cells
Prefrontal Cortex
Action Potentials
Neurons
Patch-Clamp Techniques
Cyclic AMP-Dependent Protein Kinases
Photons
Short-Term Memory
Cognition
Microscopy
Dopamine

ASJC Scopus subject areas

  • Neuroscience(all)

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D1 receptor modulation of action potential firing in a subpopulation of layer 5 pyramidal neurons in the prefrontal cortex. / Seong, Hannah J.; Carter, Adam.

In: Journal of Neuroscience, Vol. 32, No. 31, 01.08.2012, p. 10516-10521.

Research output: Contribution to journalArticle

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