Cytotoxicity of liposomal α-tocopheryl succinate towards hamster cheek pouch carcinoma (HCPC-1) cells in culture

Xinbin Gu, Joel L. Schwartz, Xiaowu Pang, Yanfei Zhou, David Sirois, Rajagopalan Sridhar

Research output: Contribution to journalArticle

Abstract

There is compelling evidence for the cancer chemopreventive effects of vitamin E and related compounds. Of all the vitamin E derivatives that have been investigated to date, vitamin E acid succinate is the most effective anti-cancer agent. This report describes the preparation and testing of liposomal formulation of mono α-tocopheryl ester of succinic acid (α-TOS) for cytotoxicity against hamster cheek pouch carcinoma cell line (HCPC-1). Small unilamellar vesicles (SUV) of phosphatidylcholine incorporating 70 μM α-TOS were superior to α-TOS alone or SUV without incorporated α-TOS, as inducers of apoptosis in HCPC-1 cells. Liposomal α-TOS perturbed the lipid structure in cells, promoted apoptosis, and decreased cell viability. The mechanism of action of α-TOS appears to involve membrane damage and induction of ceramide mediated apoptosis.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalCancer Letters
Volume239
Issue number2
DOIs
StatePublished - Aug 8 2006

Fingerprint

Cheek
Succinic Acid
Vitamin E
Cricetinae
Unilamellar Liposomes
Cell Culture Techniques
Apoptosis
Carcinoma
Ceramides
Phosphatidylcholines
Neoplasms
Cell Survival
Esters
Lipids
Cell Line
Acids
Membranes

Keywords

  • Apoptosis
  • Cancer therapy
  • Ceramide
  • Ganglioside GD3
  • Vitamin E succinate

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Cytotoxicity of liposomal α-tocopheryl succinate towards hamster cheek pouch carcinoma (HCPC-1) cells in culture. / Gu, Xinbin; Schwartz, Joel L.; Pang, Xiaowu; Zhou, Yanfei; Sirois, David; Sridhar, Rajagopalan.

In: Cancer Letters, Vol. 239, No. 2, 08.08.2006, p. 281-291.

Research output: Contribution to journalArticle

Gu, Xinbin ; Schwartz, Joel L. ; Pang, Xiaowu ; Zhou, Yanfei ; Sirois, David ; Sridhar, Rajagopalan. / Cytotoxicity of liposomal α-tocopheryl succinate towards hamster cheek pouch carcinoma (HCPC-1) cells in culture. In: Cancer Letters. 2006 ; Vol. 239, No. 2. pp. 281-291.
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