Cytotoxic T lymphocyte recognition of class I H-2 antigens after DNA-mediated gene transfer

Carol Reiss, G. A. Evans, C. Murre, D. H. Margulies, J. G. Seidman, S. J. Burakoff

Research output: Contribution to journalArticle

Abstract

Cytotoxic T lymphocyte (CTL) recognition sites on class I major histocompatibility complex molecules have been investigated by several laboratories by using cloned genes expressed on mouse L cells by DNA-mediated gene transfer. Recombinant genes, constructed by restriction endonuclease treatment of cloned H-2D(d) and L(d) genes and exchange of the N and Cl exons (exon shuffling) have provided an additional tool. These hybrid H-2 molecules expressed on L cells have been used as targets to achieve more precise localization of site(s) recognized by allospecific and virus-specific CTLs. CTL systems were chosen that limit recognition to either the D(d) or L(d) alloantigen or to virus and D(d) or L(d) complexes. Using this approach, we were able to map essential restricting site(s) to the N and/or Cl domains. Additional evidence is presented that the cytoplasmic tail of H-2 may be involved in interactions with some viral antigens and effect the formation of an immunogenic complex.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalFederation Proceedings
Volume43
Issue number2
StatePublished - 1984

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H-2 Antigens
Histocompatibility Antigens Class I
Cytotoxic T-Lymphocytes
DNA
Genes
Exons
Viruses
Isoantigens
Viral Antigens
DNA Restriction Enzymes
Major Histocompatibility Complex
Tail

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Reiss, C., Evans, G. A., Murre, C., Margulies, D. H., Seidman, J. G., & Burakoff, S. J. (1984). Cytotoxic T lymphocyte recognition of class I H-2 antigens after DNA-mediated gene transfer. Federation Proceedings, 43(2), 271-275.

Cytotoxic T lymphocyte recognition of class I H-2 antigens after DNA-mediated gene transfer. / Reiss, Carol; Evans, G. A.; Murre, C.; Margulies, D. H.; Seidman, J. G.; Burakoff, S. J.

In: Federation Proceedings, Vol. 43, No. 2, 1984, p. 271-275.

Research output: Contribution to journalArticle

Reiss, C, Evans, GA, Murre, C, Margulies, DH, Seidman, JG & Burakoff, SJ 1984, 'Cytotoxic T lymphocyte recognition of class I H-2 antigens after DNA-mediated gene transfer', Federation Proceedings, vol. 43, no. 2, pp. 271-275.
Reiss C, Evans GA, Murre C, Margulies DH, Seidman JG, Burakoff SJ. Cytotoxic T lymphocyte recognition of class I H-2 antigens after DNA-mediated gene transfer. Federation Proceedings. 1984;43(2):271-275.
Reiss, Carol ; Evans, G. A. ; Murre, C. ; Margulies, D. H. ; Seidman, J. G. ; Burakoff, S. J. / Cytotoxic T lymphocyte recognition of class I H-2 antigens after DNA-mediated gene transfer. In: Federation Proceedings. 1984 ; Vol. 43, No. 2. pp. 271-275.
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