Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarian schmidtea mediterranea

Dhiru Bansal, Jahnavi Kulkarni, Kavana Nadahalli, Vairavan Lakshmanan, Srikar Krishna, Vidyanand Sasidharan, Jini Geo, Shilpa Dilipkumar, Renu Pasricha, Akash Gulyani, Srikala Raghavan, Dasaradhi Palakodeti

Research output: Contribution to journalArticle

Abstract

Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including zfp-1, are translationally regulated by SMED-PABPC2. Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.

Original languageEnglish (US)
Pages (from-to)3066-3079
Number of pages14
JournalDevelopment (Cambridge)
Volume144
Issue number17
DOIs
StatePublished - Sep 1 2017

Fingerprint

Poly(A)-Binding Proteins
Planarians
Regeneration
Maintenance
Wound Healing
Stem Cells
Polyribosomes
Epidermis
Wounds and Injuries

Keywords

  • Epidermis
  • Neoblast
  • Planaria
  • Poly (A)-binding proteins
  • Regeneration

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Bansal, D., Kulkarni, J., Nadahalli, K., Lakshmanan, V., Krishna, S., Sasidharan, V., ... Palakodeti, D. (2017). Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarian schmidtea mediterranea. Development (Cambridge), 144(17), 3066-3079. https://doi.org/10.1242/dev.152942

Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarian schmidtea mediterranea. / Bansal, Dhiru; Kulkarni, Jahnavi; Nadahalli, Kavana; Lakshmanan, Vairavan; Krishna, Srikar; Sasidharan, Vidyanand; Geo, Jini; Dilipkumar, Shilpa; Pasricha, Renu; Gulyani, Akash; Raghavan, Srikala; Palakodeti, Dasaradhi.

In: Development (Cambridge), Vol. 144, No. 17, 01.09.2017, p. 3066-3079.

Research output: Contribution to journalArticle

Bansal, D, Kulkarni, J, Nadahalli, K, Lakshmanan, V, Krishna, S, Sasidharan, V, Geo, J, Dilipkumar, S, Pasricha, R, Gulyani, A, Raghavan, S & Palakodeti, D 2017, 'Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarian schmidtea mediterranea', Development (Cambridge), vol. 144, no. 17, pp. 3066-3079. https://doi.org/10.1242/dev.152942
Bansal, Dhiru ; Kulkarni, Jahnavi ; Nadahalli, Kavana ; Lakshmanan, Vairavan ; Krishna, Srikar ; Sasidharan, Vidyanand ; Geo, Jini ; Dilipkumar, Shilpa ; Pasricha, Renu ; Gulyani, Akash ; Raghavan, Srikala ; Palakodeti, Dasaradhi. / Cytoplasmic poly (A)-binding protein critically regulates epidermal maintenance and turnover in the planarian schmidtea mediterranea. In: Development (Cambridge). 2017 ; Vol. 144, No. 17. pp. 3066-3079.
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