Cypin

A cytosolic regulator of PSD-95 postsynaptic targeting

Bonnie L. Firestein, Jay E. Brenman, Chiye Aoki, Ana M. Sanchez-Perez, Alaa El Din Ei-Husseini, David S. Bredt

Research output: Contribution to journalArticle

Abstract

Postsynaptic density 95 (PSD-95/SAP-90) is a membrane associated guanylate kinase (GK) PDZ protein that scaffolds glutamate receptors and associated signaling networks at excitatory synapses. Affinity chromatography identifies cypin as a major PSD-95-binding protein in brain extracts. Cypin is homologous to a family of hydrolyric bacterial enzymes and shares some similarity with collapsin response mediator protein (CRMP), a cytoplasmic mediator of semaphorin III signalling. Cypin is discretely expressed in neurons and is polarized to basal membranes in intestinal epithelial cells. Overexpression of cypin in hippocampal neurons specifically perturbs postsynaptic trafficking of PSD-95 and SAP-102, an effect not produced by overexpression of other PDZ ligands. In fact, PSD-95 can induce postsynaptic clustering of an otherwise diffusely localized K+ channel, Kv1.4. By regulating postsynaptic protein sorting, cypin may influence synaptic development and plasticity.

Original languageEnglish (US)
Pages (from-to)659-672
Number of pages14
JournalNeuron
Volume24
Issue number3
StatePublished - Nov 1999

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Semaphorin-3A
Guanylate Kinases
Semaphorins
Post-Synaptic Density
Neurons
Neuronal Plasticity
Glutamate Receptors
Protein Transport
Affinity Chromatography
Synapses
Cluster Analysis
Carrier Proteins
Proteins
Epithelial Cells
Ligands
Membranes
Brain
Enzymes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Firestein, B. L., Brenman, J. E., Aoki, C., Sanchez-Perez, A. M., Ei-Husseini, A. E. D., & Bredt, D. S. (1999). Cypin: A cytosolic regulator of PSD-95 postsynaptic targeting. Neuron, 24(3), 659-672.

Cypin : A cytosolic regulator of PSD-95 postsynaptic targeting. / Firestein, Bonnie L.; Brenman, Jay E.; Aoki, Chiye; Sanchez-Perez, Ana M.; Ei-Husseini, Alaa El Din; Bredt, David S.

In: Neuron, Vol. 24, No. 3, 11.1999, p. 659-672.

Research output: Contribution to journalArticle

Firestein, BL, Brenman, JE, Aoki, C, Sanchez-Perez, AM, Ei-Husseini, AED & Bredt, DS 1999, 'Cypin: A cytosolic regulator of PSD-95 postsynaptic targeting', Neuron, vol. 24, no. 3, pp. 659-672.
Firestein BL, Brenman JE, Aoki C, Sanchez-Perez AM, Ei-Husseini AED, Bredt DS. Cypin: A cytosolic regulator of PSD-95 postsynaptic targeting. Neuron. 1999 Nov;24(3):659-672.
Firestein, Bonnie L. ; Brenman, Jay E. ; Aoki, Chiye ; Sanchez-Perez, Ana M. ; Ei-Husseini, Alaa El Din ; Bredt, David S. / Cypin : A cytosolic regulator of PSD-95 postsynaptic targeting. In: Neuron. 1999 ; Vol. 24, No. 3. pp. 659-672.
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