Cyclo-oxygenase products of arachidonic acid metabolism in rat osteoblasts in culture

R. D. Nolan, N. C. Partridge, H. M. Godfrey, T. J. Martin

Research output: Contribution to journalArticle

Abstract

The metabolism of arachidonic acid to its cyclo-oxygenase products was studied in monolayer cultures of osteoblast-rich rat calvarial cells and of clonal cell lines from a rat osteogenic sarcoma, enriched in the osteoblast phenotype. Prostanoids were measured by radioimmunoassay after extraction of media and fractionation by high pressure liquid chromatography. In both normal and malignant osteoblasts the major cyclo-oxygenase product was 6-oxo-prostaglandin F, the hydration product of prostacyclin, with lesser amounts of prostaglandin E2 and prostaglandin F. No significant thromboxane B2 was detected. Prostaglandins are thought to have a local role in the regulation of bone resorption. These results point to the possible importance of prostacyclin either in bone resorption or in some other local function, e.g., regulation of bone blood flow.

Original languageEnglish (US)
Pages (from-to)294-297
Number of pages4
JournalCalcified Tissue International
Volume35
Issue number1
DOIs
StatePublished - Dec 1 1983

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Keywords

  • Bone resorption
  • Osteoblasts
  • Prostaglandins

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

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