Crystal structure of a paired domain-DNA complex at 2.5 Å resolution reveals structural basis for pax developmental mutations

Wenqing Xu, Mark A. Rould, Susie Jun, Claude Desplan, Carl O. Pabo

Research output: Contribution to journalArticle

Abstract

The 2.5 Å resolution structure of a cocrystal containing the paired domain from the Drosophila paired (prd) protein and a 15 bp site shows structually independent N-terminal and C-terminal subdomains. Each of these domains contains a helical region resembling the homeodomain and the Hin recombinase. The N-terminal domain makes extensive DNA contacts, using a novel β turn motif that binds in the minor groove and a helixturn-helix unit with a docking arrangement surprisingly similar to that of the γ repressor. The C-terminal domain is not essential for prd binding and does not contact the optimized site. All known developmental missense mutations in the paired box of mammalian Pax genes map to the N-terminal subdomain, and most of them are found at the protein-DNA interface.

Original languageEnglish (US)
Pages (from-to)639-650
Number of pages12
JournalCell
Volume80
Issue number4
StatePublished - 1995

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Crystal structure
Mutation
DNA
Missense Mutation
Genes
Proteins
Drosophila paired protein
Hin recombinase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Crystal structure of a paired domain-DNA complex at 2.5 Å resolution reveals structural basis for pax developmental mutations. / Xu, Wenqing; Rould, Mark A.; Jun, Susie; Desplan, Claude; Pabo, Carl O.

In: Cell, Vol. 80, No. 4, 1995, p. 639-650.

Research output: Contribution to journalArticle

Xu, Wenqing ; Rould, Mark A. ; Jun, Susie ; Desplan, Claude ; Pabo, Carl O. / Crystal structure of a paired domain-DNA complex at 2.5 Å resolution reveals structural basis for pax developmental mutations. In: Cell. 1995 ; Vol. 80, No. 4. pp. 639-650.
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