Crystal structure of a continuous three-dimensional DNA lattice

Paul J. Paukstelis, Jacek Nowakowski, Jens J. Birktoft, Nadrian C. Seeman

Research output: Contribution to journalArticle

Abstract

DNA has proved to be a versatile material for the rational design and assembly of nanometer scale objects. Here we report the crystal structure of a continuous three-dimensional DNA lattice formed by the self-assembly of a DNA 13-mer. The structure consists of stacked layers of parallel helices with adjacent layers linked through parallel-stranded base pairing. The hexagonal lattice geometry contains solvent channels that appear large enough to allow 3′-linked guest molecules into the crystal. We have successfully used these parallel base pairs to design and produce crystals with greatly enlarged solvent channels. This lattice may have applications as a molecular scaffold for structure determination of guest molecules, as a molecular sieve, or in the assembly of molecular electronics. Predictable non-Watson-Crick base pairs, like those described here, may present a new tool in structural DNA nanotechnology.

Original languageEnglish (US)
Pages (from-to)1119-1126
Number of pages8
JournalChemistry and Biology
Volume11
Issue number8
DOIs
StatePublished - Aug 1 2004

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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