Critical role of IRF1 and BATF in forming chromatin landscape during type 1 regulatory cell differentiation

Katarzyna Karwacz, Emily R. Miraldi, Maria Pokrovskii, Asaf Madi, Nir Yosef, Ivo Wortman, Xi Chen, Aaron Watters, Nicholas Carriero, Amit Awasthi, Aviv Regev, Richard Bonneau, Dan Littman, Vijay K. Kuchroo

Research output: Contribution to journalArticle

Abstract

Type 1 regulatory T cells (Tr1 cells) are induced by interleukin-27 (IL-27) and have critical roles in the control of autoimmunity and resolution of inflammation. We found that the transcription factors IRF1 and BATF were induced early on after treatment with IL-27 and were required for the differentiation and function of Tr1 cells in vitro and in vivo. Epigenetic and transcriptional analyses revealed that both transcription factors influenced chromatin accessibility and expression of the genes required for Tr1 cell function. IRF1 and BATF deficiencies uniquely altered the chromatin landscape, suggesting that these factors serve a pioneering function during Tr1 cell differentiation.

Original languageEnglish (US)
Pages (from-to)412-421
Number of pages10
JournalNature Immunology
Volume18
Issue number4
DOIs
StatePublished - Mar 22 2017

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Karwacz, K., Miraldi, E. R., Pokrovskii, M., Madi, A., Yosef, N., Wortman, I., Chen, X., Watters, A., Carriero, N., Awasthi, A., Regev, A., Bonneau, R., Littman, D., & Kuchroo, V. K. (2017). Critical role of IRF1 and BATF in forming chromatin landscape during type 1 regulatory cell differentiation. Nature Immunology, 18(4), 412-421. https://doi.org/10.1038/ni.3683