Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

A. Syed, L. Mangano, P. Mao, J. Han, Rafael Song

Research output: Contribution to journalArticle

Abstract

In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes. This journal is

Original languageEnglish (US)
Pages (from-to)4455-4460
Number of pages6
JournalLab on a Chip
Volume14
Issue number23
DOIs
StatePublished - Dec 7 2014

Fingerprint

Nanofluidics
Biomolecules
Microchannels
Silicon Dioxide
Self assembly
Silica
Ions
Membranes
DNA
Proteins
Polarization

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules. / Syed, A.; Mangano, L.; Mao, P.; Han, J.; Song, Rafael.

In: Lab on a Chip, Vol. 14, No. 23, 07.12.2014, p. 4455-4460.

Research output: Contribution to journalArticle

@article{e91708fa866140b7af015dd7296d672c,
title = "Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules",
abstract = "In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes. This journal is",
author = "A. Syed and L. Mangano and P. Mao and J. Han and Rafael Song",
year = "2014",
month = "12",
day = "7",
doi = "10.1039/c4lc00895b",
language = "English (US)",
volume = "14",
pages = "4455--4460",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "23",

}

TY - JOUR

T1 - Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

AU - Syed, A.

AU - Mangano, L.

AU - Mao, P.

AU - Han, J.

AU - Song, Rafael

PY - 2014/12/7

Y1 - 2014/12/7

N2 - In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes. This journal is

AB - In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes. This journal is

UR - http://www.scopus.com/inward/record.url?scp=84908272720&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908272720&partnerID=8YFLogxK

U2 - 10.1039/c4lc00895b

DO - 10.1039/c4lc00895b

M3 - Article

VL - 14

SP - 4455

EP - 4460

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 23

ER -