Coordinated hydrolysis explains the mechanical behavior of kinesin

Charles Peskin, G. Oster, M. Irving, S. Leibler, B. Brenner, J. Howard, D. Hackney, D. Smith, K. Johnson

Research output: Contribution to journalArticle

Abstract

The two-headed motor protein kinesin hydrolyzes nucleotide to move unidirectionally along its microtubule track at speeds up to 1000 nm/s (Saxton et al., 1988) and develops forces in excess of 5 pN (Hunt et al., 1994; Svoboda et al., 1994a). Individual kinesin molecules have been studied recently in vitro, and their behavior has been characterized in terms of force-velocity curves and variance measurements (Svoboda and Block, 1994a; Svoboda et al., 1994b). We present a model for force generation in kinesin in which the ATP hydrolysis reactions are coordinated with the relative positions of the two heads. The model explains the experimental data and permits us to study the relative roles of Brownian motion and elastic deformation in the motor mechanism of kinesin.

Original languageEnglish (US)
JournalBiophysical Journal
Volume68
Issue number4 SUPPL.
StatePublished - 1995

Fingerprint

Kinesin
Hydrolysis
Microtubules
Theoretical Models
Nucleotides
Adenosine Triphosphate
Head
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Peskin, C., Oster, G., Irving, M., Leibler, S., Brenner, B., Howard, J., ... Johnson, K. (1995). Coordinated hydrolysis explains the mechanical behavior of kinesin. Biophysical Journal, 68(4 SUPPL.).

Coordinated hydrolysis explains the mechanical behavior of kinesin. / Peskin, Charles; Oster, G.; Irving, M.; Leibler, S.; Brenner, B.; Howard, J.; Hackney, D.; Smith, D.; Johnson, K.

In: Biophysical Journal, Vol. 68, No. 4 SUPPL., 1995.

Research output: Contribution to journalArticle

Peskin, C, Oster, G, Irving, M, Leibler, S, Brenner, B, Howard, J, Hackney, D, Smith, D & Johnson, K 1995, 'Coordinated hydrolysis explains the mechanical behavior of kinesin', Biophysical Journal, vol. 68, no. 4 SUPPL..
Peskin C, Oster G, Irving M, Leibler S, Brenner B, Howard J et al. Coordinated hydrolysis explains the mechanical behavior of kinesin. Biophysical Journal. 1995;68(4 SUPPL.).
Peskin, Charles ; Oster, G. ; Irving, M. ; Leibler, S. ; Brenner, B. ; Howard, J. ; Hackney, D. ; Smith, D. ; Johnson, K. / Coordinated hydrolysis explains the mechanical behavior of kinesin. In: Biophysical Journal. 1995 ; Vol. 68, No. 4 SUPPL.
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