Cooperative dimerization of paired class homeo domains on DNA

D. Wilson, G. Sheng, T. Lecuit, N. Dostatni, C. Desplan

Research output: Contribution to journalArticle

Abstract

Homeo domain-containing proteins mediate many transcriptional processes in eukaryotes. Because nearly all animal homeo proteins are believed to bind to short, highly related DNA sequences, the basis for their high specificity of action is not understood. We show that cooperative dimerization on palindromic DNA sequences can provide increased specificity to one of the three major classes of homeo domains, the Paired/Pax class. The 60-amino- acid homeo domains from this class contain sufficient information to bind cooperatively as homo- and heterodimers to palindromic DNA sequences; that is, the binding of one homeo domain molecule can increase the affinity of a second molecule by up to 300-fold. Different members of the Paired (Prd) class of homeo domains prefer different spacings between half-sites, as determined by the ninth amino acid residue of the recognition helix. In addition, this residue determines the identity of the base pairs at the center of the palindromic sites, as well as the magnitude of the cooperative interaction. The cooperative dimerization of homeo domains in the Prd class distinguishes them from other classes, whereas binding-site configuration and sequence specificity allow for distinctions within this class.

Original languageEnglish (US)
Pages (from-to)2120-2134
Number of pages15
JournalGenes & development
Volume7
Issue number11
StatePublished - 1993

Fingerprint

Dimerization
DNA
Homeodomain Proteins
Amino Acids
Eukaryota
Base Pairing
Binding Sites
Proteins

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Wilson, D., Sheng, G., Lecuit, T., Dostatni, N., & Desplan, C. (1993). Cooperative dimerization of paired class homeo domains on DNA. Genes & development, 7(11), 2120-2134.

Cooperative dimerization of paired class homeo domains on DNA. / Wilson, D.; Sheng, G.; Lecuit, T.; Dostatni, N.; Desplan, C.

In: Genes & development, Vol. 7, No. 11, 1993, p. 2120-2134.

Research output: Contribution to journalArticle

Wilson, D, Sheng, G, Lecuit, T, Dostatni, N & Desplan, C 1993, 'Cooperative dimerization of paired class homeo domains on DNA', Genes & development, vol. 7, no. 11, pp. 2120-2134.
Wilson D, Sheng G, Lecuit T, Dostatni N, Desplan C. Cooperative dimerization of paired class homeo domains on DNA. Genes & development. 1993;7(11):2120-2134.
Wilson, D. ; Sheng, G. ; Lecuit, T. ; Dostatni, N. ; Desplan, C. / Cooperative dimerization of paired class homeo domains on DNA. In: Genes & development. 1993 ; Vol. 7, No. 11. pp. 2120-2134.
@article{af97f3e0d57847b383950945e0bf692b,
title = "Cooperative dimerization of paired class homeo domains on DNA",
abstract = "Homeo domain-containing proteins mediate many transcriptional processes in eukaryotes. Because nearly all animal homeo proteins are believed to bind to short, highly related DNA sequences, the basis for their high specificity of action is not understood. We show that cooperative dimerization on palindromic DNA sequences can provide increased specificity to one of the three major classes of homeo domains, the Paired/Pax class. The 60-amino- acid homeo domains from this class contain sufficient information to bind cooperatively as homo- and heterodimers to palindromic DNA sequences; that is, the binding of one homeo domain molecule can increase the affinity of a second molecule by up to 300-fold. Different members of the Paired (Prd) class of homeo domains prefer different spacings between half-sites, as determined by the ninth amino acid residue of the recognition helix. In addition, this residue determines the identity of the base pairs at the center of the palindromic sites, as well as the magnitude of the cooperative interaction. The cooperative dimerization of homeo domains in the Prd class distinguishes them from other classes, whereas binding-site configuration and sequence specificity allow for distinctions within this class.",
author = "D. Wilson and G. Sheng and T. Lecuit and N. Dostatni and C. Desplan",
year = "1993",
language = "English (US)",
volume = "7",
pages = "2120--2134",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "11",

}

TY - JOUR

T1 - Cooperative dimerization of paired class homeo domains on DNA

AU - Wilson, D.

AU - Sheng, G.

AU - Lecuit, T.

AU - Dostatni, N.

AU - Desplan, C.

PY - 1993

Y1 - 1993

N2 - Homeo domain-containing proteins mediate many transcriptional processes in eukaryotes. Because nearly all animal homeo proteins are believed to bind to short, highly related DNA sequences, the basis for their high specificity of action is not understood. We show that cooperative dimerization on palindromic DNA sequences can provide increased specificity to one of the three major classes of homeo domains, the Paired/Pax class. The 60-amino- acid homeo domains from this class contain sufficient information to bind cooperatively as homo- and heterodimers to palindromic DNA sequences; that is, the binding of one homeo domain molecule can increase the affinity of a second molecule by up to 300-fold. Different members of the Paired (Prd) class of homeo domains prefer different spacings between half-sites, as determined by the ninth amino acid residue of the recognition helix. In addition, this residue determines the identity of the base pairs at the center of the palindromic sites, as well as the magnitude of the cooperative interaction. The cooperative dimerization of homeo domains in the Prd class distinguishes them from other classes, whereas binding-site configuration and sequence specificity allow for distinctions within this class.

AB - Homeo domain-containing proteins mediate many transcriptional processes in eukaryotes. Because nearly all animal homeo proteins are believed to bind to short, highly related DNA sequences, the basis for their high specificity of action is not understood. We show that cooperative dimerization on palindromic DNA sequences can provide increased specificity to one of the three major classes of homeo domains, the Paired/Pax class. The 60-amino- acid homeo domains from this class contain sufficient information to bind cooperatively as homo- and heterodimers to palindromic DNA sequences; that is, the binding of one homeo domain molecule can increase the affinity of a second molecule by up to 300-fold. Different members of the Paired (Prd) class of homeo domains prefer different spacings between half-sites, as determined by the ninth amino acid residue of the recognition helix. In addition, this residue determines the identity of the base pairs at the center of the palindromic sites, as well as the magnitude of the cooperative interaction. The cooperative dimerization of homeo domains in the Prd class distinguishes them from other classes, whereas binding-site configuration and sequence specificity allow for distinctions within this class.

UR - http://www.scopus.com/inward/record.url?scp=0027332476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027332476&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 2120

EP - 2134

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 11

ER -