Controlling airborne cues to study small animal navigation

Marc Gershow, Matthew Berck, Dennis Mathew, Linjiao Luo, Elizabeth A. Kane, John R. Carlson, Aravinthan D T Samuel

    Research output: Contribution to journalArticle

    Abstract

    Small animals such as nematodes and insects analyze airborne chemical cues to infer the direction of favorable and noxious locations. In these animals, the study of navigational behavior evoked by airborne cues has been limited by the difficulty of precisely controlling stimuli. We present a system that can be used to deliver gaseous stimuli in defined spatial and temporal patterns to freely moving small animals. We used this apparatus, in combination with machine-vision algorithms, to assess and quantify navigational decision making of Drosophila melanogaster larvae in response to ethyl acetate (a volatile attractant) and carbon dioxide (a gaseous repellant).

    Original languageEnglish (US)
    Pages (from-to)290-296
    Number of pages7
    JournalNature Methods
    Volume9
    Issue number3
    DOIs
    StatePublished - Mar 2012

    Fingerprint

    Cues
    Animals
    Navigation
    Drosophila melanogaster
    Carbon Dioxide
    Computer vision
    Larva
    Insects
    Decision Making
    Decision making
    ethyl acetate
    Direction compound

    ASJC Scopus subject areas

    • Biotechnology
    • Molecular Biology
    • Biochemistry
    • Cell Biology

    Cite this

    Gershow, M., Berck, M., Mathew, D., Luo, L., Kane, E. A., Carlson, J. R., & Samuel, A. D. T. (2012). Controlling airborne cues to study small animal navigation. Nature Methods, 9(3), 290-296. https://doi.org/10.1038/nmeth.1853

    Controlling airborne cues to study small animal navigation. / Gershow, Marc; Berck, Matthew; Mathew, Dennis; Luo, Linjiao; Kane, Elizabeth A.; Carlson, John R.; Samuel, Aravinthan D T.

    In: Nature Methods, Vol. 9, No. 3, 03.2012, p. 290-296.

    Research output: Contribution to journalArticle

    Gershow, M, Berck, M, Mathew, D, Luo, L, Kane, EA, Carlson, JR & Samuel, ADT 2012, 'Controlling airborne cues to study small animal navigation', Nature Methods, vol. 9, no. 3, pp. 290-296. https://doi.org/10.1038/nmeth.1853
    Gershow M, Berck M, Mathew D, Luo L, Kane EA, Carlson JR et al. Controlling airborne cues to study small animal navigation. Nature Methods. 2012 Mar;9(3):290-296. https://doi.org/10.1038/nmeth.1853
    Gershow, Marc ; Berck, Matthew ; Mathew, Dennis ; Luo, Linjiao ; Kane, Elizabeth A. ; Carlson, John R. ; Samuel, Aravinthan D T. / Controlling airborne cues to study small animal navigation. In: Nature Methods. 2012 ; Vol. 9, No. 3. pp. 290-296.
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