Control of helical handedness in DNA and PNA nanostructures.

Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Mark M. Green, Rosangela Marchelli

Research output: Contribution to journalArticle

Abstract

Helical handedness and the twist and tilt parameters of the base pairs in duplex DNA can be affected by base sequence variation and change in environmental conditions as occurs in the transformation between right-handed B-DNA and left-handed Z-DNA. For duplexes of DNA with oligonucleotide analogs such as peptide nucleic acids (PNAs), less is known about the effects on structure such as the base pair twist and tilt parameters and handedness. However, in PNA:PNA duplexes, the absence of chiral information determining helical handedness allows the relationship between preferred helical handedness and structural design to be manipulated and, therefore, better understood. In this chapter, we report a protocol for switching between B- and Z-DNA:DNA duplexes, and the experimental procedures for obtaining right- or left-handed PNA:PNA duplexes.

Original languageEnglish (US)
Pages (from-to)79-92
Number of pages14
JournalMethods in Molecular Biology
Volume749
StatePublished - 2011

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Peptide Nucleic Acids
Functional Laterality
Nanostructures
Z-Form DNA
DNA
Base Pairing
B-Form DNA
Oligonucleotides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Corradini, R., Tedeschi, T., Sforza, S., Green, M. M., & Marchelli, R. (2011). Control of helical handedness in DNA and PNA nanostructures. Methods in Molecular Biology, 749, 79-92.

Control of helical handedness in DNA and PNA nanostructures. / Corradini, Roberto; Tedeschi, Tullia; Sforza, Stefano; Green, Mark M.; Marchelli, Rosangela.

In: Methods in Molecular Biology, Vol. 749, 2011, p. 79-92.

Research output: Contribution to journalArticle

Corradini, R, Tedeschi, T, Sforza, S, Green, MM & Marchelli, R 2011, 'Control of helical handedness in DNA and PNA nanostructures.', Methods in Molecular Biology, vol. 749, pp. 79-92.
Corradini R, Tedeschi T, Sforza S, Green MM, Marchelli R. Control of helical handedness in DNA and PNA nanostructures. Methods in Molecular Biology. 2011;749:79-92.
Corradini, Roberto ; Tedeschi, Tullia ; Sforza, Stefano ; Green, Mark M. ; Marchelli, Rosangela. / Control of helical handedness in DNA and PNA nanostructures. In: Methods in Molecular Biology. 2011 ; Vol. 749. pp. 79-92.
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