Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential

Rafael Song, Jongyoon Han

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel continuous pI-based sorting technique has been developed for proteins and peptides. It can continuously process samples at a relatively high flow rate of 10L/min and requires no ampholytes. The electrophoretic field required for the separation of biomolecules is generated by the diffusion of different buffer ionic species in-situ at the laminar flow junction. Using this diffusion potential, a pI-based separation of proteins and peptides has been successfully demonstrated. The resolution of the sorting technique and its efficiency were characterized by fluorescence intensity measurement, as well as by 2D protein gel electrophoresis.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1025-1027
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Fingerprint

Sorting
Microfluidics
Peptides
Proteins
Buffers
Biomolecules
Electrophoresis
Laminar flow
Gels
Fluorescence
Flow rate

Keywords

  • Continuous freeflow electrophoresis
  • Diffusion potential
  • Protein separation
  • Transverse IEF

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Song, R., & Han, J. (2005). Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 1025-1027). Transducer Research Foundation.

Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. / Song, Rafael; Han, Jongyoon.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 1025-1027.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Song, R & Han, J 2005, Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 1025-1027, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.
Song R, Han J. Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1. Transducer Research Foundation. 2005. p. 1025-1027
Song, Rafael ; Han, Jongyoon. / Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. pp. 1025-1027
@inproceedings{f0fe8afb99624baba09552671931e04d,
title = "Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential",
abstract = "A novel continuous pI-based sorting technique has been developed for proteins and peptides. It can continuously process samples at a relatively high flow rate of 10L/min and requires no ampholytes. The electrophoretic field required for the separation of biomolecules is generated by the diffusion of different buffer ionic species in-situ at the laminar flow junction. Using this diffusion potential, a pI-based separation of proteins and peptides has been successfully demonstrated. The resolution of the sorting technique and its efficiency were characterized by fluorescence intensity measurement, as well as by 2D protein gel electrophoresis.",
keywords = "Continuous freeflow electrophoresis, Diffusion potential, Protein separation, Transverse IEF",
author = "Rafael Song and Jongyoon Han",
year = "2005",
month = "1",
day = "1",
language = "English (US)",
isbn = "0974361119",
volume = "1",
pages = "1025--1027",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference",
publisher = "Transducer Research Foundation",

}

TY - GEN

T1 - Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential

AU - Song, Rafael

AU - Han, Jongyoon

PY - 2005/1/1

Y1 - 2005/1/1

N2 - A novel continuous pI-based sorting technique has been developed for proteins and peptides. It can continuously process samples at a relatively high flow rate of 10L/min and requires no ampholytes. The electrophoretic field required for the separation of biomolecules is generated by the diffusion of different buffer ionic species in-situ at the laminar flow junction. Using this diffusion potential, a pI-based separation of proteins and peptides has been successfully demonstrated. The resolution of the sorting technique and its efficiency were characterized by fluorescence intensity measurement, as well as by 2D protein gel electrophoresis.

AB - A novel continuous pI-based sorting technique has been developed for proteins and peptides. It can continuously process samples at a relatively high flow rate of 10L/min and requires no ampholytes. The electrophoretic field required for the separation of biomolecules is generated by the diffusion of different buffer ionic species in-situ at the laminar flow junction. Using this diffusion potential, a pI-based separation of proteins and peptides has been successfully demonstrated. The resolution of the sorting technique and its efficiency were characterized by fluorescence intensity measurement, as well as by 2D protein gel electrophoresis.

KW - Continuous freeflow electrophoresis

KW - Diffusion potential

KW - Protein separation

KW - Transverse IEF

UR - http://www.scopus.com/inward/record.url?scp=84902435536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902435536&partnerID=8YFLogxK

M3 - Conference contribution

SN - 0974361119

SN - 9780974361116

VL - 1

SP - 1025

EP - 1027

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference

PB - Transducer Research Foundation

ER -