Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential

Rafael Song, Jongyoon Han

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    A novel continuous pI-based sorting technique has been developed for proteins and peptides. It can continuously process samples at a relatively high flow rate of 10L/min and requires no ampholytes. The electrophoretic field required for the separation of biomolecules is generated by the diffusion of different buffer ionic species in-situ at the laminar flow junction. Using this diffusion potential, a pI-based separation of proteins and peptides has been successfully demonstrated. The resolution of the sorting technique and its efficiency were characterized by fluorescence intensity measurement, as well as by 2D protein gel electrophoresis.

    Original languageEnglish (US)
    Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
    Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherTransducer Research Foundation
    Pages1025-1027
    Number of pages3
    Volume1
    ISBN (Print)0974361119, 9780974361116
    StatePublished - Jan 1 2005
    Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
    Duration: Oct 9 2005Oct 13 2005

    Other

    Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
    CountryUnited States
    CityBoston, MA
    Period10/9/0510/13/05

    Fingerprint

    Sorting
    Microfluidics
    Peptides
    Proteins
    Buffers
    Biomolecules
    Electrophoresis
    Laminar flow
    Gels
    Fluorescence
    Flow rate

    Keywords

    • Continuous freeflow electrophoresis
    • Diffusion potential
    • Protein separation
    • Transverse IEF

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Song, R., & Han, J. (2005). Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 1025-1027). Transducer Research Foundation.

    Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. / Song, Rafael; Han, Jongyoon.

    Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 1025-1027.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Song, R & Han, J 2005, Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 1025-1027, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.
    Song R, Han J. Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1. Transducer Research Foundation. 2005. p. 1025-1027
    Song, Rafael ; Han, Jongyoon. / Continuous pI-based sorting of proteins and peptides in a microfluidic chip using diffusion potential. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. pp. 1025-1027
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