Contact inhibition of locomotion in vivo controls neural crest directional migration

Carlos Carmona Fontaine, Helen K. Matthews, Sei Kuriyama, Mauricio Moreno, Graham A. Dunn, Maddy Parsons, Claudio D. Stern, Roberto Mayor

Research output: Contribution to journalArticle

Abstract

Contact inhibition of locomotion was discovered by Abercrombie more than 50 years ago and describes the behaviour of fibroblast cells confronting each other in vitro, where they retract their protrusions and change direction on contact. Its failure was suggested to contribute to malignant invasion. However, the molecular basis of contact inhibition of locomotion and whether it also occurs in vivo are still unknown. Here we show that neural crest cells, a highly migratory and multipotent embryonic cell population, whose behaviour has been likened to malignant invasion, demonstrate contact inhibition of locomotion both in vivo and in vitro, and that this accounts for their directional migration. When two migrating neural crest cells meet, they stop, collapse their protrusions and change direction. In contrast, when a neural crest cell meets another cell type, it fails to display contact inhibition of locomotion; instead, it invades the other tissue, in the same manner as metastatic cancer cells. We show that inhibition of non-canonical Wnt signalling abolishes both contact inhibition of locomotion and the directionality of neural crest migration. Wnt-signalling members localize at the site of cell contact, leading to activation of RhoA in this region. These results provide the first example of contact inhibition of locomotion in vivo, provide an explanation for coherent directional migration of groups of cells and establish a previously unknown role for non-canonical Wnt signalling.

Original languageEnglish (US)
Pages (from-to)957-961
Number of pages5
JournalNature
Volume456
Issue number7224
DOIs
StatePublished - Dec 18 2008

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Contact Inhibition
Neural Crest
Locomotion
Cell Movement
Fibroblasts
Population
Neoplasms

ASJC Scopus subject areas

  • General

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Carmona Fontaine, C., Matthews, H. K., Kuriyama, S., Moreno, M., Dunn, G. A., Parsons, M., ... Mayor, R. (2008). Contact inhibition of locomotion in vivo controls neural crest directional migration. Nature, 456(7224), 957-961. https://doi.org/10.1038/nature07441

Contact inhibition of locomotion in vivo controls neural crest directional migration. / Carmona Fontaine, Carlos; Matthews, Helen K.; Kuriyama, Sei; Moreno, Mauricio; Dunn, Graham A.; Parsons, Maddy; Stern, Claudio D.; Mayor, Roberto.

In: Nature, Vol. 456, No. 7224, 18.12.2008, p. 957-961.

Research output: Contribution to journalArticle

Carmona Fontaine, C, Matthews, HK, Kuriyama, S, Moreno, M, Dunn, GA, Parsons, M, Stern, CD & Mayor, R 2008, 'Contact inhibition of locomotion in vivo controls neural crest directional migration', Nature, vol. 456, no. 7224, pp. 957-961. https://doi.org/10.1038/nature07441
Carmona Fontaine C, Matthews HK, Kuriyama S, Moreno M, Dunn GA, Parsons M et al. Contact inhibition of locomotion in vivo controls neural crest directional migration. Nature. 2008 Dec 18;456(7224):957-961. https://doi.org/10.1038/nature07441
Carmona Fontaine, Carlos ; Matthews, Helen K. ; Kuriyama, Sei ; Moreno, Mauricio ; Dunn, Graham A. ; Parsons, Maddy ; Stern, Claudio D. ; Mayor, Roberto. / Contact inhibition of locomotion in vivo controls neural crest directional migration. In: Nature. 2008 ; Vol. 456, No. 7224. pp. 957-961.
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