Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus

Cornells Murre, Carol Reiss, Carmelo Bernabeu, Lan Bo Chen, Steven J. Burakoff, J. G. Seidman

Research output: Contribution to journalArticle

Abstract

A mouse major histocompatibility antigen (H-2) gene, encoding a novel H-2Ld molecule lacking its intracytoplasmic domain, has been constructed and introduced into mouse L-cells. The novel H-2 molecule is found on the surface of the transfected cells at the same level as L-cells transfected with the native H-2Ld gene. Allo- and influenza-specific cytotoxic T lymphocytes can recognize the truncated H-2 gene product nearly as efficiently as the normal H-2Ld gene product. However, vesicular stomatitis virus-specific cytotoxic T lymphocytes recognize the truncated H-2Ld molecule less efficiently than the complete H-2Ld product. The rate of capping of the truncated H-2Ld molecule was investigated and found to be the same as that of the complete H-2Ld gene product.

Original languageEnglish (US)
Pages (from-to)432-436
Number of pages5
JournalNature
Volume307
Issue number5950
DOIs
StatePublished - 1984

Fingerprint

Genes
Cytotoxic T-Lymphocytes
Vesicular Stomatitis
Histocompatibility Antigens
Human Influenza
Viruses

ASJC Scopus subject areas

  • General

Cite this

Murre, C., Reiss, C., Bernabeu, C., Chen, L. B., Burakoff, S. J., & Seidman, J. G. (1984). Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus. Nature, 307(5950), 432-436. https://doi.org/10.1038/307432a0

Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus. / Murre, Cornells; Reiss, Carol; Bernabeu, Carmelo; Chen, Lan Bo; Burakoff, Steven J.; Seidman, J. G.

In: Nature, Vol. 307, No. 5950, 1984, p. 432-436.

Research output: Contribution to journalArticle

Murre, C, Reiss, C, Bernabeu, C, Chen, LB, Burakoff, SJ & Seidman, JG 1984, 'Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus', Nature, vol. 307, no. 5950, pp. 432-436. https://doi.org/10.1038/307432a0
Murre, Cornells ; Reiss, Carol ; Bernabeu, Carmelo ; Chen, Lan Bo ; Burakoff, Steven J. ; Seidman, J. G. / Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus. In: Nature. 1984 ; Vol. 307, No. 5950. pp. 432-436.
@article{5107e9b09ca049ecb3bb95626fa5b094,
title = "Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus",
abstract = "A mouse major histocompatibility antigen (H-2) gene, encoding a novel H-2Ld molecule lacking its intracytoplasmic domain, has been constructed and introduced into mouse L-cells. The novel H-2 molecule is found on the surface of the transfected cells at the same level as L-cells transfected with the native H-2Ld gene. Allo- and influenza-specific cytotoxic T lymphocytes can recognize the truncated H-2 gene product nearly as efficiently as the normal H-2Ld gene product. However, vesicular stomatitis virus-specific cytotoxic T lymphocytes recognize the truncated H-2Ld molecule less efficiently than the complete H-2Ld product. The rate of capping of the truncated H-2Ld molecule was investigated and found to be the same as that of the complete H-2Ld gene product.",
author = "Cornells Murre and Carol Reiss and Carmelo Bernabeu and Chen, {Lan Bo} and Burakoff, {Steven J.} and Seidman, {J. G.}",
year = "1984",
doi = "10.1038/307432a0",
language = "English (US)",
volume = "307",
pages = "432--436",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5950",

}

TY - JOUR

T1 - Construction, expression and recognition of an H - 2 molecule lacking its carboxyl terminus

AU - Murre, Cornells

AU - Reiss, Carol

AU - Bernabeu, Carmelo

AU - Chen, Lan Bo

AU - Burakoff, Steven J.

AU - Seidman, J. G.

PY - 1984

Y1 - 1984

N2 - A mouse major histocompatibility antigen (H-2) gene, encoding a novel H-2Ld molecule lacking its intracytoplasmic domain, has been constructed and introduced into mouse L-cells. The novel H-2 molecule is found on the surface of the transfected cells at the same level as L-cells transfected with the native H-2Ld gene. Allo- and influenza-specific cytotoxic T lymphocytes can recognize the truncated H-2 gene product nearly as efficiently as the normal H-2Ld gene product. However, vesicular stomatitis virus-specific cytotoxic T lymphocytes recognize the truncated H-2Ld molecule less efficiently than the complete H-2Ld product. The rate of capping of the truncated H-2Ld molecule was investigated and found to be the same as that of the complete H-2Ld gene product.

AB - A mouse major histocompatibility antigen (H-2) gene, encoding a novel H-2Ld molecule lacking its intracytoplasmic domain, has been constructed and introduced into mouse L-cells. The novel H-2 molecule is found on the surface of the transfected cells at the same level as L-cells transfected with the native H-2Ld gene. Allo- and influenza-specific cytotoxic T lymphocytes can recognize the truncated H-2 gene product nearly as efficiently as the normal H-2Ld gene product. However, vesicular stomatitis virus-specific cytotoxic T lymphocytes recognize the truncated H-2Ld molecule less efficiently than the complete H-2Ld product. The rate of capping of the truncated H-2Ld molecule was investigated and found to be the same as that of the complete H-2Ld gene product.

UR - http://www.scopus.com/inward/record.url?scp=0021240283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021240283&partnerID=8YFLogxK

U2 - 10.1038/307432a0

DO - 10.1038/307432a0

M3 - Article

VL - 307

SP - 432

EP - 436

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5950

ER -