Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans

P. Goncharoff, Julie Yip, H. Wang, H. C. Schreiner, J. A. Pai, D. Furgang, R. H. Stevens, D. H. Figurski, D. H. Fine

Research output: Contribution to journalArticle

Abstract

The first example of conjugal transfer of DNA from Escherichia coli to the periodontal pathogen Actinobacillus actinomycetemcomitans is presented. Derivatives of the incompatibility group P (IncP) plasmid RK2 successfully transferred from an E. coli donor to an A. actinomycetemcomitans recipient. The resulting A. actinomycetemcomitans transconjugants transferred the plasmids back to E. coli recipients. The IncP transfer functions were also used in trans to mobilize the IncQ plasmid pBK1 from E. coli to A. actinomycetemcomitans. The IncP and IncQ plasmids both transferred into A. actinomycetemcomitans at high frequencies (0.3 to 0.5 transconjugants per donor) and showed no gross deletions, insertions, or rearrangements. Determinations of MICs of various antibiotics for the A. actinomycetemcomitans transconjugant strains demonstrated the expression of ampicillin, chloramphenicol, and kanamycin resistance determinants.

Original languageEnglish (US)
Pages (from-to)3544-3547
Number of pages4
JournalInfection and Immunity
Volume61
Issue number8
StatePublished - 1993

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Aggregatibacter actinomycetemcomitans
Host Specificity
Plasmids
Escherichia coli
Chloramphenicol Resistance
Kanamycin Resistance
Ampicillin Resistance
Anti-Bacterial Agents
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Goncharoff, P., Yip, J., Wang, H., Schreiner, H. C., Pai, J. A., Furgang, D., ... Fine, D. H. (1993). Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans. Infection and Immunity, 61(8), 3544-3547.

Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans. / Goncharoff, P.; Yip, Julie; Wang, H.; Schreiner, H. C.; Pai, J. A.; Furgang, D.; Stevens, R. H.; Figurski, D. H.; Fine, D. H.

In: Infection and Immunity, Vol. 61, No. 8, 1993, p. 3544-3547.

Research output: Contribution to journalArticle

Goncharoff, P, Yip, J, Wang, H, Schreiner, HC, Pai, JA, Furgang, D, Stevens, RH, Figurski, DH & Fine, DH 1993, 'Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans', Infection and Immunity, vol. 61, no. 8, pp. 3544-3547.
Goncharoff, P. ; Yip, Julie ; Wang, H. ; Schreiner, H. C. ; Pai, J. A. ; Furgang, D. ; Stevens, R. H. ; Figurski, D. H. ; Fine, D. H. / Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans. In: Infection and Immunity. 1993 ; Vol. 61, No. 8. pp. 3544-3547.
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AU - Yip, Julie

AU - Wang, H.

AU - Schreiner, H. C.

AU - Pai, J. A.

AU - Furgang, D.

AU - Stevens, R. H.

AU - Figurski, D. H.

AU - Fine, D. H.

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N2 - The first example of conjugal transfer of DNA from Escherichia coli to the periodontal pathogen Actinobacillus actinomycetemcomitans is presented. Derivatives of the incompatibility group P (IncP) plasmid RK2 successfully transferred from an E. coli donor to an A. actinomycetemcomitans recipient. The resulting A. actinomycetemcomitans transconjugants transferred the plasmids back to E. coli recipients. The IncP transfer functions were also used in trans to mobilize the IncQ plasmid pBK1 from E. coli to A. actinomycetemcomitans. The IncP and IncQ plasmids both transferred into A. actinomycetemcomitans at high frequencies (0.3 to 0.5 transconjugants per donor) and showed no gross deletions, insertions, or rearrangements. Determinations of MICs of various antibiotics for the A. actinomycetemcomitans transconjugant strains demonstrated the expression of ampicillin, chloramphenicol, and kanamycin resistance determinants.

AB - The first example of conjugal transfer of DNA from Escherichia coli to the periodontal pathogen Actinobacillus actinomycetemcomitans is presented. Derivatives of the incompatibility group P (IncP) plasmid RK2 successfully transferred from an E. coli donor to an A. actinomycetemcomitans recipient. The resulting A. actinomycetemcomitans transconjugants transferred the plasmids back to E. coli recipients. The IncP transfer functions were also used in trans to mobilize the IncQ plasmid pBK1 from E. coli to A. actinomycetemcomitans. The IncP and IncQ plasmids both transferred into A. actinomycetemcomitans at high frequencies (0.3 to 0.5 transconjugants per donor) and showed no gross deletions, insertions, or rearrangements. Determinations of MICs of various antibiotics for the A. actinomycetemcomitans transconjugant strains demonstrated the expression of ampicillin, chloramphenicol, and kanamycin resistance determinants.

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