Conformational properties of B-Z junctions in DNA

Min Lu, Qiu Guo, Neville R. Kallenbach, Richard D. Sheardy

Research output: Contribution to journalArticle

Abstract

The structural consequences of specific base sequences in DNA can exert a strong influence on the function of DNA. It has previously been reported that the presence of multiple B-Z conformational junctions in constructed DNA oligomers results in unusually enhanced electrophoretic gel mobilities of these oligomers [Winkle, S. A., & Sheardy, R. D. (1990) Biochemistry 29, 6514-6521]. In order to investigate this phenomenon further, we designed and synthesized several DNA oligomers capable of pure Z or B-Z junction formation for polyacrylamide gel electrophoresis studies. The results indicate that both pure Z-DNA and polymorphic B-Z-DNA oligomers exhibit unusual gel migratory properties. The results of gel mobility studies in the absence and presence of cobalt hexamine indicate that a B-Z junction corresponds to a stiff bend of the helix axis, with two or more conformers accessible at the junction site. This is a different bend and mechanism than that in oligo(A) tracts.

Original languageEnglish (US)
Pages (from-to)4712-4719
Number of pages8
JournalBiochemistry
Volume31
Issue number19
StatePublished - 1992

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Oligomers
Z-Form DNA
Gels
DNA
Methenamine
Biochemistry
Cobalt
Electrophoresis
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lu, M., Guo, Q., Kallenbach, N. R., & Sheardy, R. D. (1992). Conformational properties of B-Z junctions in DNA. Biochemistry, 31(19), 4712-4719.

Conformational properties of B-Z junctions in DNA. / Lu, Min; Guo, Qiu; Kallenbach, Neville R.; Sheardy, Richard D.

In: Biochemistry, Vol. 31, No. 19, 1992, p. 4712-4719.

Research output: Contribution to journalArticle

Lu, M, Guo, Q, Kallenbach, NR & Sheardy, RD 1992, 'Conformational properties of B-Z junctions in DNA', Biochemistry, vol. 31, no. 19, pp. 4712-4719.
Lu M, Guo Q, Kallenbach NR, Sheardy RD. Conformational properties of B-Z junctions in DNA. Biochemistry. 1992;31(19):4712-4719.
Lu, Min ; Guo, Qiu ; Kallenbach, Neville R. ; Sheardy, Richard D. / Conformational properties of B-Z junctions in DNA. In: Biochemistry. 1992 ; Vol. 31, No. 19. pp. 4712-4719.
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