Confocal microscopy of cardiac myocytes

Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, Louis Terracio

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Detailed methods are provided for the preparation and confocal imaging of cardiac myocyte development and differentiation. Examples include protocols for the analysis of cultured myocytes as well as vibratome sections of hearts from embryonic and adult tissue. Techniques include routine labeling of F-actin with phalloidin as well as multiple labeling protocols for colocalization studies and cell volume analysis.

Original languageEnglish (US)
Title of host publicationConfocal Microscopy: Methods and Protocols
PublisherHumana Press Inc.
Pages185-199
Number of pages15
Volume1075
ISBN (Print)9781588293510
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1075
ISSN (Print)10643745

Fingerprint

Phalloidine
Cell Size
Cardiac Myocytes
Confocal Microscopy
Muscle Cells
Actins

Keywords

  • Cardiac myocyte
  • Cardiovascular
  • Confocal microscopy
  • Image analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Price, R. L., Haley, S. T., Bullard, T., Davis, J., Borg, T. K., & Terracio, L. (2014). Confocal microscopy of cardiac myocytes. In Confocal Microscopy: Methods and Protocols (Vol. 1075, pp. 185-199). (Methods in Molecular Biology; Vol. 1075). Humana Press Inc.. https://doi.org/10.1007/978-1-60761-847-8_8

Confocal microscopy of cardiac myocytes. / Price, Robert L.; Haley, Stephen T.; Bullard, Tara; Davis, Jeffrey; Borg, Thomas K.; Terracio, Louis.

Confocal Microscopy: Methods and Protocols. Vol. 1075 Humana Press Inc., 2014. p. 185-199 (Methods in Molecular Biology; Vol. 1075).

Research output: Chapter in Book/Report/Conference proceedingChapter

Price, RL, Haley, ST, Bullard, T, Davis, J, Borg, TK & Terracio, L 2014, Confocal microscopy of cardiac myocytes. in Confocal Microscopy: Methods and Protocols. vol. 1075, Methods in Molecular Biology, vol. 1075, Humana Press Inc., pp. 185-199. https://doi.org/10.1007/978-1-60761-847-8_8
Price RL, Haley ST, Bullard T, Davis J, Borg TK, Terracio L. Confocal microscopy of cardiac myocytes. In Confocal Microscopy: Methods and Protocols. Vol. 1075. Humana Press Inc. 2014. p. 185-199. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60761-847-8_8
Price, Robert L. ; Haley, Stephen T. ; Bullard, Tara ; Davis, Jeffrey ; Borg, Thomas K. ; Terracio, Louis. / Confocal microscopy of cardiac myocytes. Confocal Microscopy: Methods and Protocols. Vol. 1075 Humana Press Inc., 2014. pp. 185-199 (Methods in Molecular Biology).
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