Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array

Danni Wang, Andres Mansisidor, Gayathri Prabhakar, Andreas Hochwagen

Research output: Contribution to journalArticle

Abstract

Repetitive DNA arrays are important structural features of eukaryotic genomes that are often heterochromatinized to suppress repeat instability. It is unclear, however, whether all repeats within an array are equally subject to heterochromatin formation and gene silencing. Here, we show that in starving Saccharomyces cerevisiae, silencing of reporter genes within the ribosomal DNA (rDNA) array is less pronounced in outer repeats compared with inner repeats. This position effect is linked to the starvation-induced contraction of the nucleolus. We show that the chromatin regulators condensin and Hmo1 redistribute within the rDNA upon starvation; that Hmo1, like condensin, is required for nucleolar contraction; and that the position effect partially depends on both proteins. Starvation-induced nucleolar contraction and differential desilencing of the outer rDNA repeats may provide a mechanism to activate rDNA-encoded RNAPII transcription units without causing general rDNA instability.

Original languageEnglish (US)
Pages (from-to)1010-1017
Number of pages8
JournalCell Reports
Volume14
Issue number5
DOIs
StatePublished - Feb 9 2016

Fingerprint

Starvation
Ribosomal DNA
Oligonucleotide Array Sequence Analysis
Genes
Heterochromatin
Gene Silencing
Transcription
Reporter Genes
Yeast
Chromatin
Saccharomyces cerevisiae
condensin complexes
Genome
DNA
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. / Wang, Danni; Mansisidor, Andres; Prabhakar, Gayathri; Hochwagen, Andreas.

In: Cell Reports, Vol. 14, No. 5, 09.02.2016, p. 1010-1017.

Research output: Contribution to journalArticle

Wang, Danni ; Mansisidor, Andres ; Prabhakar, Gayathri ; Hochwagen, Andreas. / Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. In: Cell Reports. 2016 ; Vol. 14, No. 5. pp. 1010-1017.
@article{b8e9eb884370473681a2acb32a08e364,
title = "Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array",
abstract = "Repetitive DNA arrays are important structural features of eukaryotic genomes that are often heterochromatinized to suppress repeat instability. It is unclear, however, whether all repeats within an array are equally subject to heterochromatin formation and gene silencing. Here, we show that in starving Saccharomyces cerevisiae, silencing of reporter genes within the ribosomal DNA (rDNA) array is less pronounced in outer repeats compared with inner repeats. This position effect is linked to the starvation-induced contraction of the nucleolus. We show that the chromatin regulators condensin and Hmo1 redistribute within the rDNA upon starvation; that Hmo1, like condensin, is required for nucleolar contraction; and that the position effect partially depends on both proteins. Starvation-induced nucleolar contraction and differential desilencing of the outer rDNA repeats may provide a mechanism to activate rDNA-encoded RNAPII transcription units without causing general rDNA instability.",
author = "Danni Wang and Andres Mansisidor and Gayathri Prabhakar and Andreas Hochwagen",
year = "2016",
month = "2",
day = "9",
doi = "10.1016/j.celrep.2016.01.005",
language = "English (US)",
volume = "14",
pages = "1010--1017",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array

AU - Wang, Danni

AU - Mansisidor, Andres

AU - Prabhakar, Gayathri

AU - Hochwagen, Andreas

PY - 2016/2/9

Y1 - 2016/2/9

N2 - Repetitive DNA arrays are important structural features of eukaryotic genomes that are often heterochromatinized to suppress repeat instability. It is unclear, however, whether all repeats within an array are equally subject to heterochromatin formation and gene silencing. Here, we show that in starving Saccharomyces cerevisiae, silencing of reporter genes within the ribosomal DNA (rDNA) array is less pronounced in outer repeats compared with inner repeats. This position effect is linked to the starvation-induced contraction of the nucleolus. We show that the chromatin regulators condensin and Hmo1 redistribute within the rDNA upon starvation; that Hmo1, like condensin, is required for nucleolar contraction; and that the position effect partially depends on both proteins. Starvation-induced nucleolar contraction and differential desilencing of the outer rDNA repeats may provide a mechanism to activate rDNA-encoded RNAPII transcription units without causing general rDNA instability.

AB - Repetitive DNA arrays are important structural features of eukaryotic genomes that are often heterochromatinized to suppress repeat instability. It is unclear, however, whether all repeats within an array are equally subject to heterochromatin formation and gene silencing. Here, we show that in starving Saccharomyces cerevisiae, silencing of reporter genes within the ribosomal DNA (rDNA) array is less pronounced in outer repeats compared with inner repeats. This position effect is linked to the starvation-induced contraction of the nucleolus. We show that the chromatin regulators condensin and Hmo1 redistribute within the rDNA upon starvation; that Hmo1, like condensin, is required for nucleolar contraction; and that the position effect partially depends on both proteins. Starvation-induced nucleolar contraction and differential desilencing of the outer rDNA repeats may provide a mechanism to activate rDNA-encoded RNAPII transcription units without causing general rDNA instability.

UR - http://www.scopus.com/inward/record.url?scp=84957963272&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957963272&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2016.01.005

DO - 10.1016/j.celrep.2016.01.005

M3 - Article

VL - 14

SP - 1010

EP - 1017

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 5

ER -