Composition analysis of poly(ethylene glycol)-poly(L-lactide) diblock copolymer studied by two-dimensional column chromatography

Dean Lee, Iwao Teraoka, Tomoko Fujiwara, Yoshiharu Kimura

Research output: Contribution to journalArticle

Abstract

We used two-dimensional column chromatography to analyze the composition of a sample of presumably a diblock copolymer of poly(ethylene glycol) (PEG) and poly(L-lactide) synthesized from monomethoxy-terminated PEG. The first dimension of the separation is phase fluctuation chromatography to prepare fractions that contain various components of the copolymer in different ratios. The second dimension is size-exclusion chromatography, NMR, and HPLC at the critical condition of PEG. The PEG initiator has small amounts of diol-terminated dimeric components. We found that the copolymer sample contains a triblock copolymer and low-molecular-mass components in addition to the main part of the diblock copolymer. The SEC chromatograms show that the main part consists of two components with distinct peak lengths for the PLLA block. The low-molecular-mass components have a broad distribution in chemical composition. Phase fluctuation chromatography enriched the triblock copolymer and the diblock copolymer with the longer PLLA block in early fractions when the column was packed with carboxymethyl-modified porous silica. When the porous medium was PLLA-grafted silica, size exclusion dominated, but the low-molecular-mass components were separated according to their chemical composition.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalJournal of Chromatography A
Volume966
Issue number1-2
DOIs
StatePublished - Aug 9 2002

Fingerprint

Column chromatography
Ethylene Glycol
Polyethylene glycols
Block copolymers
Chromatography
Molecular mass
Silicon Dioxide
Chemical analysis
Copolymers
Gel Chromatography
High Pressure Liquid Chromatography
Size exclusion chromatography
Phase separation
Porous materials
Nuclear magnetic resonance
poly(lactide)

Keywords

  • Diblock copolymer
  • Phase fluctuation chromatography
  • Poly(ethylene glycol)
  • Poly(L-lactide)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Composition analysis of poly(ethylene glycol)-poly(L-lactide) diblock copolymer studied by two-dimensional column chromatography. / Lee, Dean; Teraoka, Iwao; Fujiwara, Tomoko; Kimura, Yoshiharu.

In: Journal of Chromatography A, Vol. 966, No. 1-2, 09.08.2002, p. 41-51.

Research output: Contribution to journalArticle

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