Composite microfiltration membranes imprinted with cAMP

Victor Kochkodan, Nidal Hilal, Phill J. Windsor, Edward Lester

    Research output: Contribution to journalArticle

    Abstract

    Composite microfiltration membranes covered with a thin layer of molecularly imprinted polymer (MIP) selective to adenosine 3′:5′-cyclic monophosphate (cAMP) were obtained and their separation properties were studied. MIP layers were prepared using photoinitiated copolymerization of dimethylaminoethyl methacrylate (DMAEM) as a functional monomer and trimethylopropane trimethacrylate (TRIM) as a crosslinker in the presence of cAMP as template in ethanol/water mixture. Blank membranes were prepared under the same conditions, but without cAMP. It was found out that pH of aquecous solution of the template has an effect on the binding of cAMP with MIP membranes. It was concluded that the ability of MIP membranes to bind cAMP is a result of both ionic interactions between charged dimethylamino groups of polymer matrix and the phosphorous residue of cAMP molecule and the specific shape of recognizing sites. These sites are complementary to cAMP in terms of three dimensional shape as well as correct position of functional groups involved in the template binding. This paper shows that the binding capability of MIP membranes can be adjusted by varying the values of degree of modification (DM). Atomic Force Microscopy (AFM) and Scanning Force Microscopy (SEM) were used to visualise surfaces and cross sections of membranes to gain better understanding in the analysis of MIP layer deposited on membranes.

    Original languageEnglish (US)
    Pages (from-to)463-468
    Number of pages6
    JournalChemical Engineering and Technology
    Volume26
    Issue number4
    DOIs
    StatePublished - Apr 1 2003

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    Microfiltration
    Polymers
    Membranes
    Composite materials
    Atomic force microscopy
    Methacrylates
    Polymer matrix
    Copolymerization
    Functional groups
    Ethanol
    Monomers
    Molecules
    Water

    ASJC Scopus subject areas

    • Chemistry(all)
    • Chemical Engineering(all)
    • Industrial and Manufacturing Engineering

    Cite this

    Composite microfiltration membranes imprinted with cAMP. / Kochkodan, Victor; Hilal, Nidal; Windsor, Phill J.; Lester, Edward.

    In: Chemical Engineering and Technology, Vol. 26, No. 4, 01.04.2003, p. 463-468.

    Research output: Contribution to journalArticle

    Kochkodan, V, Hilal, N, Windsor, PJ & Lester, E 2003, 'Composite microfiltration membranes imprinted with cAMP', Chemical Engineering and Technology, vol. 26, no. 4, pp. 463-468. https://doi.org/10.1002/ceat.200390069
    Kochkodan, Victor ; Hilal, Nidal ; Windsor, Phill J. ; Lester, Edward. / Composite microfiltration membranes imprinted with cAMP. In: Chemical Engineering and Technology. 2003 ; Vol. 26, No. 4. pp. 463-468.
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