Comparison of transfection efficiency of nonviral gene transfer reagents

Research output: Contribution to journalArticle

Abstract

This study compared six commercially available reagents (Arrest-In, ExpressFect, FuGENE HD, jetPEI, Lipofectamine 2000, and SuperFect) for gene transfection. We examined the efficiency and cytotoxicity using nine different cell lines (MC3T3-E1 mouse preosteoblasts, PT-30 human epithelial precancer cells, C3H10T1/2 mouse stem cells, MCF-7 human breast cancer cells, HeLa human cervical cancer, C2C12 mouse myoblasts, Hep G2 human hepatocellular carcinoma, 4T1 mouse mammary carcinoma, and HCT116 human colorectal carcinoma), and primary cells (HEKn human epidermal keratinocytes) with two different plasmid DNAs encoding luciferase or β-galactosidase in the presence or absence of serum. Maximal transfection efficiency in MC3T3-E1, C3H10T1/2, HeLa, C2C12, Hep G2, and HCT116 was seen using FuGENE HD, in PT-30, 4T1, and HEKn was seen using Arrest-In, and in MCF-7 was seen using jetPEI. Determination of cytotoxicity showed that the largest amount of viable cells was found after transfection with jetPEI and ExpressFect. These results suggest that FuGENE HD is the most preferred transfection reagent for many cell lines, followed by Arrest-In and jetPEI. These results may be useful for improving nonviral gene and cell therapy applications.

Original languageEnglish (US)
Pages (from-to)287-300
Number of pages14
JournalMolecular Biotechnology
Volume46
Issue number3
DOIs
StatePublished - Nov 2010

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Gene transfer
Transfection
Cells
Cytotoxicity
Genes
Galactosidases
Stem cells
Luciferases
Breast Neoplasms
Plasmids
DNA
Cell Line
Myoblasts
Cell- and Tissue-Based Therapy
Keratinocytes
HeLa Cells
Uterine Cervical Neoplasms
Genetic Therapy
Hepatocellular Carcinoma
Colorectal Neoplasms

Keywords

  • Activated-dendrimer
  • Cationic lipid
  • Gene transfection
  • Polyethylenimine
  • Polymer

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Comparison of transfection efficiency of nonviral gene transfer reagents. / Yamano, Seiichi; Dai, Jisen; Moursi, Amr.

In: Molecular Biotechnology, Vol. 46, No. 3, 11.2010, p. 287-300.

Research output: Contribution to journalArticle

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