Comparison of the kinetic effects of phospholamban phosphorylation and anti-phospholamban monoclonal antibody on the calcium pump in purified cardiac sarcoplasmic reticulum membranes

Alexander Y. Antipenko, Andrew Spielman, Massimo Sassaroli, Madeleine A. Kirchberger

Research output: Contribution to journalArticle

Abstract

Protein kinase A- (PKA-) catalyzed phosphorylation of phospholamban (PLN), the protein regulator of the cardiac Ca pump, mediates abbreviation of systole in response to β-adrenergic agonists. Investigators previously, however, have been unsuccessful in demonstrating an effect of PLN phosphorylation or anti-PLN monoclonal antibody (mAb), which is considered to mimic phosphorylation's well-known effect on K(m(Ca)), on microsomal Ca uptake at the (high) Ca 2+ concentrations found intracellularly at peak systole. We therefore compared the effects of the catalytic subunit of PKA and anti-PLN mAb on the kinetics of Ca uptake in sucrose gradient-purified cardiac microsomes. Both treatments produced a 33-44% increase in V(max(Ca)) at 25 and 37 °C, and an 11-31% decrease in K(m(Ca)) with comparable changes in Ca 2+-ATPase activity. An acceleration of E 2P decomposition upon PLN phosphorylation may contribute to the increased V(max(Ca)) of Ca uptake at 25 °C but not at 37 °C, based on measurement of the kinetics of E 2P decomposition and steady-state E 2P formation from P(i) at different temperatures. Our data document almost identical increases in V(max(Ca)) of microsomal Ca uptake with PLN phosphorylation or addition of anti-PLN mAb and hence provide insight into the kinetic mechanism of PLN's regulation of the cardiac sarcoplasmic reticulum Ca pump protein.

Original languageEnglish (US)
Pages (from-to)12903-12910
Number of pages8
JournalBiochemistry
Volume36
Issue number42
DOIs
StatePublished - Oct 21 1997

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Phosphorylation
Sarcoplasmic Reticulum
Monoclonal Antibodies
Pumps
Calcium
Membranes
Kinetics
Systole
Decomposition
Adrenergic Agonists
Microsomes
Cyclic AMP-Dependent Protein Kinases
phospholamban
Adenosine Triphosphatases
Sucrose
Catalytic Domain
Proteins
Research Personnel
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Comparison of the kinetic effects of phospholamban phosphorylation and anti-phospholamban monoclonal antibody on the calcium pump in purified cardiac sarcoplasmic reticulum membranes. / Antipenko, Alexander Y.; Spielman, Andrew; Sassaroli, Massimo; Kirchberger, Madeleine A.

In: Biochemistry, Vol. 36, No. 42, 21.10.1997, p. 12903-12910.

Research output: Contribution to journalArticle

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abstract = "Protein kinase A- (PKA-) catalyzed phosphorylation of phospholamban (PLN), the protein regulator of the cardiac Ca pump, mediates abbreviation of systole in response to β-adrenergic agonists. Investigators previously, however, have been unsuccessful in demonstrating an effect of PLN phosphorylation or anti-PLN monoclonal antibody (mAb), which is considered to mimic phosphorylation's well-known effect on K(m(Ca)), on microsomal Ca uptake at the (high) Ca 2+ concentrations found intracellularly at peak systole. We therefore compared the effects of the catalytic subunit of PKA and anti-PLN mAb on the kinetics of Ca uptake in sucrose gradient-purified cardiac microsomes. Both treatments produced a 33-44{\%} increase in V(max(Ca)) at 25 and 37 °C, and an 11-31{\%} decrease in K(m(Ca)) with comparable changes in Ca 2+-ATPase activity. An acceleration of E 2P decomposition upon PLN phosphorylation may contribute to the increased V(max(Ca)) of Ca uptake at 25 °C but not at 37 °C, based on measurement of the kinetics of E 2P decomposition and steady-state E 2P formation from P(i) at different temperatures. Our data document almost identical increases in V(max(Ca)) of microsomal Ca uptake with PLN phosphorylation or addition of anti-PLN mAb and hence provide insight into the kinetic mechanism of PLN's regulation of the cardiac sarcoplasmic reticulum Ca pump protein.",
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