Comparison of Streptococcus mutans and streptococcus sanguis receptors for human salivary agglutinin

Donald R. Demuth, Margaret S. Lammey, Margaret Huck, Edward T. Lally, Daniel Malamud

Research output: Contribution to journalArticle

Abstract

Oral streptococci vary in their susceptibility to salivary agglutinin-mediated aggregation. To understand the molecular basis of this specificity, the structure and function of receptors for agglutinin from Streptococcus mutans KPSK2 (MSL-1) and Streptococcus sanguis M5 (SSP-5) were compared. Immunological screening of an S. mutans KPSK2 genomic DNA library yielded two identical clones expressing a streptococcal protein that co-migrated with a 220 kDa peptide in SDS extracts from this organism. This protein inhibited agglutinin-mediated aggregation of S. mutans KPSK2 in a dose-dependent manner. The MSL-1 gene is homologous to the S. Mutans SpaP and pac genes although single base substitutions alter several amino acids. MSL-1 is also similar to the agglutinin receptor (SSP-5) cloned from S. sanguis M5. All three proteins, MSL-1, P1, and SSP-5 share at least one epitope since monoclonal and polyclonal anti-SSP-5 antibodies react with both MSL-1 and P1. However, other monoclonal antibodies are specific for SSP-5 and appear to react with a peptide domain exhibiting little homology to MSL-1 or P1. Sugar inhibition studies showed that agglutinin-mediated aggregation of S. mutans KPSK2 was most potently inhibited by fucose and lactose. Sialic acid, a potent inhibitor of S. sanguis aggregation, had no effect on the interaction of agglutinin with S. mutans KPSK2. These results suggest that while the MSL-1 and SSP-5 proteins are genetically and immunologically related, their specificity for binding sites on agglutinin differs.

Original languageEnglish (US)
Pages (from-to)199-211
Number of pages13
JournalMicrobial Pathogenesis
Volume9
Issue number3
DOIs
StatePublished - 1990

Fingerprint

Streptococcus sanguis
Streptococcus mutans
Agglutinins
Proteins
Fucose
Genomic Library
N-Acetylneuraminic Acid
Lactose
Streptococcus
Gene Library
Genes
Epitopes
Clone Cells
Binding Sites
Monoclonal Antibodies
Amino Acids
Peptides
Antibodies

Keywords

  • receptor
  • salivary agglutinin
  • Streptococcus mutans
  • Streptococcus sanguis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Comparison of Streptococcus mutans and streptococcus sanguis receptors for human salivary agglutinin. / Demuth, Donald R.; Lammey, Margaret S.; Huck, Margaret; Lally, Edward T.; Malamud, Daniel.

In: Microbial Pathogenesis, Vol. 9, No. 3, 1990, p. 199-211.

Research output: Contribution to journalArticle

Demuth, Donald R. ; Lammey, Margaret S. ; Huck, Margaret ; Lally, Edward T. ; Malamud, Daniel. / Comparison of Streptococcus mutans and streptococcus sanguis receptors for human salivary agglutinin. In: Microbial Pathogenesis. 1990 ; Vol. 9, No. 3. pp. 199-211.
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