Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma.

Smruti Pushalkar, Xiaojie Ji, Yihong Li, Cherry Estilo, Ramanathan Yegnanarayana, Bhuvanesh Singh, Xin Li, Deepak Saxena

Research output: Contribution to journalArticle

Abstract

Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples. DGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species/phylotypes were detected representing six phyla, Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Actinobacteria and uncultivated TM7 in non-tumor and tumor libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera were observed. Bacterial species, Streptococcus sp. oral taxon 058, Peptostreptococcus stomatis, Streptococcus salivarius, Streptococcus gordonii, Gemella haemolysans, Gemella morbillorum, Johnsonella ignava and Streptococcus parasanguinis I were highly associated with tumor site where as Granulicatella adiacens was prevalent at non-tumor site. Streptococcus intermedius was present in 70% of both non-tumor and tumor sites. The underlying changes in the bacterial diversity in the oral mucosal tissues from non-tumor and tumor sites of OSCC subjects indicated a shift in bacterial colonization. These most prevalent or unique bacterial species/phylotypes present in tumor tissues may be associated with OSCC and needs to be further investigated with a larger sample size.

Original languageEnglish (US)
Article number144
JournalBMC Microbiology
Volume12
StatePublished - 2012

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Microbiota
Squamous Cell Carcinoma
Neoplasms
Gemella
Denaturing Gradient Gel Electrophoresis
Streptococcus
Libraries
Mucous Membrane
Streptococcus intermedius
Fusobacteria
Streptococcus gordonii
Bacteroidetes
Peptostreptococcus
Proteobacteria
Aptitude
Actinobacteria
Dermatoglyphics
Bacterial Infections
Sample Size
Organism Cloning

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma. / Pushalkar, Smruti; Ji, Xiaojie; Li, Yihong; Estilo, Cherry; Yegnanarayana, Ramanathan; Singh, Bhuvanesh; Li, Xin; Saxena, Deepak.

In: BMC Microbiology, Vol. 12, 144, 2012.

Research output: Contribution to journalArticle

Pushalkar, Smruti ; Ji, Xiaojie ; Li, Yihong ; Estilo, Cherry ; Yegnanarayana, Ramanathan ; Singh, Bhuvanesh ; Li, Xin ; Saxena, Deepak. / Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma. In: BMC Microbiology. 2012 ; Vol. 12.
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