Comparative C19‐Radiosteroid Metabolism in Primary Monolayer Cultures of Epithelial Cells and Fibroblasts from Rat Ventral Prostate, Canine Prostate, and Rat Lung

PETER OFNER, RICHARD L. VENA, Louis Terracio, WILLIAM H J DOUGLAS

Research output: Contribution to journalArticle

Abstract

Stromal‐epithelial cell interactions may be important for the regulation of normal and abnormal prostatic growth. While androgen transformation by rat ventral prostate and canine prostate has been investigated in vivo and in organ culture, little is known about metabolic pathways in cultures of epithelial cells and fibroblasts. Metabolism of radioisotope‐labeled 17β‐hydroxy C19‐steroids was studied in primary cultures of highly‐enriched rat ventral prostate and canine prostate epithelial cells and fibroblasts isolated by selective attachment procedures. The fibroblasts contained little testosterone 5α‐reductase in contrast to high activity in epithelial cells. We found high levels of 5α‐androstane‐3α,17β‐diol (3α‐diol) dehydrogenase and the terminal 5α‐androstane‐3β,17β‐diol (3β‐diol) hydroxylases in both cell types; 3α‐diol was a more effective precursor of 5α‐dihydrotestosterone than was testosterone. For prostatic fibroblasts these pathways seem to be differentiated functions, since rat‐lung fibroblasts converted 3β‐diol to 5α‐dihydrotestosterone and 3α‐diol. We conclude that epithelial cells and fibroblasts make interactive contributions to prostatic androgen metabolism. 1982 American Society of Andrology

Original languageEnglish (US)
Pages (from-to)269-280
Number of pages12
JournalJournal of Andrology
Volume3
Issue number5
DOIs
StatePublished - 1982

Fingerprint

Canidae
Prostate
Fibroblasts
Epithelial Cells
Lung
Androgens
Testosterone
Organ Culture Techniques
Mixed Function Oxygenases
Metabolic Networks and Pathways
Cell Communication
Oxidoreductases
Growth

Keywords

  • 3α‐hydroxysteroid oxidoreductase
  • 3β‐hydroxy 5α‐C‐steroid hydroxylases
  • canine prostate
  • epithelial cells
  • fibroblasts
  • primary cell culture
  • rat ventral prostate
  • testosterone 5α‐reductase

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Urology

Cite this

Comparative C19‐Radiosteroid Metabolism in Primary Monolayer Cultures of Epithelial Cells and Fibroblasts from Rat Ventral Prostate, Canine Prostate, and Rat Lung. / OFNER, PETER; VENA, RICHARD L.; Terracio, Louis; DOUGLAS, WILLIAM H J.

In: Journal of Andrology, Vol. 3, No. 5, 1982, p. 269-280.

Research output: Contribution to journalArticle

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