Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning

Joshua G. Corbin, Michael Rutlin, Nicholas Gaiano, Gordon Fishell

    Research output: Contribution to journalArticle

    Abstract

    Regional patterning of the mammalian telencephalon requires the function of three homeodomain-containing transcription factors, Pax6, Gsh2 and Nkx2.1. These factors are required for the development of the dorsal, lateral and medial domains of the telencephalon, respectively. Previous work has indicated that two of the genes encoding these factors, Pax6 and Gsh2, cross-repress one another in the formation of the border between dorsal and lateral region of the telencephalon. Here, we examine whether similar interactions are responsible for the establishment of other boundaries of telencephalic gene expression. Surprisingly, despite the fact that, at specific times in development, both Pax6 and Gsh2 maintain a complementary pattern of expression with Nkx2.1, in neither case are these boundaries maintained through a similar cross-repressive mechanism. Rather, as revealed by analysis of double-mutant mice, Nkx2.1 and Gsh2 act cooperatively in many aspects to pattern the ventral telencephalon. By contrast, as indicated by both loss- and gain-of-function analysis, Gsh2 expression in the medial ganglionic eminence after E10.5 may negatively regulate Nkx2.1 dependent specification of oligodendrocytes. Therefore, both integrative and antagonistic interactions between homeodomain-containing transcription factors contribute to the patterning of the telencephalon.

    Original languageEnglish (US)
    Pages (from-to)4895-4906
    Number of pages12
    JournalDevelopment
    Volume130
    Issue number20
    DOIs
    StatePublished - Oct 1 2003

    Fingerprint

    Homeodomain Proteins
    Telencephalon
    Transcription Factors
    Median Eminence
    Oligodendroglia
    Gene Expression
    Genes

    Keywords

    • Gsh2
    • Mouse
    • Nkx2.1
    • Patterning
    • Pax6
    • Telencephalon

    ASJC Scopus subject areas

    • Anatomy
    • Cell Biology

    Cite this

    Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. / Corbin, Joshua G.; Rutlin, Michael; Gaiano, Nicholas; Fishell, Gordon.

    In: Development, Vol. 130, No. 20, 01.10.2003, p. 4895-4906.

    Research output: Contribution to journalArticle

    Corbin, Joshua G. ; Rutlin, Michael ; Gaiano, Nicholas ; Fishell, Gordon. / Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. In: Development. 2003 ; Vol. 130, No. 20. pp. 4895-4906.
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