Combinatorial chromatin dynamics foster accurate cardiopharyngeal fate choices

Claudia Racioppi, Keira A. Wiechecki, Lionel Christiaen

Research output: Contribution to journalArticle

Abstract

During embryogenesis, chromatin accessibility profiles control lineage-specific gene expression by modulating transcription, thus impacting multipotent progenitor states and subsequent fate choices. Subsets of cardiac and pharyngeal/head muscles share a common origin in the cardiopharyngeal mesoderm, but the chromatin landscapes that govern multipotent progenitors competence and early fate choices remain largely elusive. Here, we leveraged the simplicity of the chordate model Ciona to profile chromatin accessibility through stereotyped transitions from naive Mesp+ mesoderm to distinct fate-restricted heart and pharyngeal muscle precursors. An FGF-Foxf pathway acts in multipotent progenitors to establish cardiopharyngeal specific patterns of accessibility, which govern later heart vs. pharyngeal muscle-specific expression profiles, demonstrating extensive spatiotemporal decoupling between early cardiopharyngeal enhancer accessibility and late cell-type-specific activity. We found that multiple cis-regulatory elements, with distinct chromatin accessibility profiles and motif compositions, are required to activate Ebf and Tbx1/10, two key determinants of cardiopharyngeal fate choices. We propose that these “combined enhancers” foster spatially and temporally accurate fate choices, by increasing the repertoire of regulatory inputs that control gene expression, through either accessibility and/or activity.

Original languageEnglish (US)
Article numbere49921
JournaleLife
Volume8
DOIs
StatePublished - Nov 2019

Fingerprint

Pharyngeal Muscles
Chromatin
Muscle
Mesoderm
Gene expression
Chordata
Gene Expression
Transcription
Mental Competency
Embryonic Development
Myocardium
Head
Chemical analysis

Keywords

  • ATAC-seq
  • Chordate
  • CRISPR/Cas9
  • Enhancer
  • Head Muscle
  • Heart
  • Pharyngeal Muscle
  • Transcription

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Combinatorial chromatin dynamics foster accurate cardiopharyngeal fate choices. / Racioppi, Claudia; Wiechecki, Keira A.; Christiaen, Lionel.

In: eLife, Vol. 8, e49921, 11.2019.

Research output: Contribution to journalArticle

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