Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy

Yanyan Liu, Taiki Yanagishima, Arran Curran, Kazem V. Edmond, Stefano Sacanna, Roel P.A. Dullens

Research output: Contribution to journalArticle

Abstract

We describe the synthesis and application of 3-(trimethoxysilyl)propyl methacrylate (TPM) particles as a colloidal model system for three-dimensional (3D) confocal scanning laser microscopy. The effect of the initial TPM concentration on the growth and polydispersity of the particles and a recently developed solvent transfer method to disperse particles in a refractive index and density-matching solvent mixture are reviewed and discussed. To fully characterize the system as a colloidal model, we measure the pair potential between the TPM particles directly using optical tweezers. Finally, we use 3D confocal microscopy to image a sedimentation-diffusion equilibrium of TPM particles to characterize the phase behavior and particle dynamics through successful detection and tracking of all particles in the field of view.

Original languageEnglish (US)
Pages (from-to)7962-7969
Number of pages8
JournalLangmuir
Volume35
Issue number24
DOIs
StatePublished - Jun 18 2019

Fingerprint

Confocal microscopy
microscopy
Optical tweezers
Polydispersity
Phase behavior
Sedimentation
Refractive index
Microscopic examination
Scanning
Lasers
laser microscopy
3-(trimethoxysilyl)propyl methacrylate
field of view
refractivity
scanning
synthesis

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Liu, Y., Yanagishima, T., Curran, A., Edmond, K. V., Sacanna, S., & Dullens, R. P. A. (2019). Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy. Langmuir, 35(24), 7962-7969. https://doi.org/10.1021/acs.langmuir.9b00963

Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy. / Liu, Yanyan; Yanagishima, Taiki; Curran, Arran; Edmond, Kazem V.; Sacanna, Stefano; Dullens, Roel P.A.

In: Langmuir, Vol. 35, No. 24, 18.06.2019, p. 7962-7969.

Research output: Contribution to journalArticle

Liu, Y, Yanagishima, T, Curran, A, Edmond, KV, Sacanna, S & Dullens, RPA 2019, 'Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy', Langmuir, vol. 35, no. 24, pp. 7962-7969. https://doi.org/10.1021/acs.langmuir.9b00963
Liu Y, Yanagishima T, Curran A, Edmond KV, Sacanna S, Dullens RPA. Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy. Langmuir. 2019 Jun 18;35(24):7962-7969. https://doi.org/10.1021/acs.langmuir.9b00963
Liu, Yanyan ; Yanagishima, Taiki ; Curran, Arran ; Edmond, Kazem V. ; Sacanna, Stefano ; Dullens, Roel P.A. / Colloidal Organosilica Spheres for Three-Dimensional Confocal Microscopy. In: Langmuir. 2019 ; Vol. 35, No. 24. pp. 7962-7969.
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