Collier/OLF/EBF-Dependent Transcriptional Dynamics Control Pharyngeal Muscle Specification from Primed Cardiopharyngeal Progenitors

Florian Razy-Krajka, Karen Lam, Wei Wang, Alberto Stolfi, Marine Joly, Richard Bonneau, Lionel Christiaen

Research output: Contribution to journalArticle

Abstract

In vertebrates, pluripotent pharyngeal mesoderm progenitors produce the cardiac precursors of the second heart field as well as the branchiomeric head muscles and associated stem cells. However, the mechanisms underlying the transition from multipotent progenitors to distinct muscle precursors remain obscured by the complexity of vertebrate embryos. Using Ciona intestinalis as a simple chordate model, we show that bipotent cardiopharyngeal progenitors are primed to activate both heart and pharyngeal muscle transcriptional programs, which progressively become restricted to corresponding precursors. The transcription factor COE (Collier/OLF/EBF) orchestrates the transition to pharyngeal muscle fate both by promoting an MRF-associated myogenic program in myoblasts and by maintaining an undifferentiated state in their sister cells through Notch-mediated lateral inhibition. The latter are stem cell-like muscle precursors that form most of the juvenile pharyngeal muscles. We discuss the implications of our findings for the development and evolution of the chordate cardiopharyngeal mesoderm.

Original languageEnglish (US)
JournalDevelopmental Cell
Volume29
Issue number3
DOIs
StatePublished - May 12 2014

Fingerprint

Pharyngeal Muscles
Chordata
Muscle
Mesoderm
Specifications
Muscles
Vertebrates
Stem Cells
Ciona intestinalis
Myoblasts
Stem cells
Myocardium
Transcription Factors
Embryonic Structures
Head

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Collier/OLF/EBF-Dependent Transcriptional Dynamics Control Pharyngeal Muscle Specification from Primed Cardiopharyngeal Progenitors. / Razy-Krajka, Florian; Lam, Karen; Wang, Wei; Stolfi, Alberto; Joly, Marine; Bonneau, Richard; Christiaen, Lionel.

In: Developmental Cell, Vol. 29, No. 3, 12.05.2014.

Research output: Contribution to journalArticle

@article{9c686bce434e4b098b9a7f97f3978a6a,
title = "Collier/OLF/EBF-Dependent Transcriptional Dynamics Control Pharyngeal Muscle Specification from Primed Cardiopharyngeal Progenitors",
abstract = "In vertebrates, pluripotent pharyngeal mesoderm progenitors produce the cardiac precursors of the second heart field as well as the branchiomeric head muscles and associated stem cells. However, the mechanisms underlying the transition from multipotent progenitors to distinct muscle precursors remain obscured by the complexity of vertebrate embryos. Using Ciona intestinalis as a simple chordate model, we show that bipotent cardiopharyngeal progenitors are primed to activate both heart and pharyngeal muscle transcriptional programs, which progressively become restricted to corresponding precursors. The transcription factor COE (Collier/OLF/EBF) orchestrates the transition to pharyngeal muscle fate both by promoting an MRF-associated myogenic program in myoblasts and by maintaining an undifferentiated state in their sister cells through Notch-mediated lateral inhibition. The latter are stem cell-like muscle precursors that form most of the juvenile pharyngeal muscles. We discuss the implications of our findings for the development and evolution of the chordate cardiopharyngeal mesoderm.",
author = "Florian Razy-Krajka and Karen Lam and Wei Wang and Alberto Stolfi and Marine Joly and Richard Bonneau and Lionel Christiaen",
year = "2014",
month = "5",
day = "12",
doi = "10.1016/j.devcel.2014.04.001",
language = "English (US)",
volume = "29",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - Collier/OLF/EBF-Dependent Transcriptional Dynamics Control Pharyngeal Muscle Specification from Primed Cardiopharyngeal Progenitors

AU - Razy-Krajka, Florian

AU - Lam, Karen

AU - Wang, Wei

AU - Stolfi, Alberto

AU - Joly, Marine

AU - Bonneau, Richard

AU - Christiaen, Lionel

PY - 2014/5/12

Y1 - 2014/5/12

N2 - In vertebrates, pluripotent pharyngeal mesoderm progenitors produce the cardiac precursors of the second heart field as well as the branchiomeric head muscles and associated stem cells. However, the mechanisms underlying the transition from multipotent progenitors to distinct muscle precursors remain obscured by the complexity of vertebrate embryos. Using Ciona intestinalis as a simple chordate model, we show that bipotent cardiopharyngeal progenitors are primed to activate both heart and pharyngeal muscle transcriptional programs, which progressively become restricted to corresponding precursors. The transcription factor COE (Collier/OLF/EBF) orchestrates the transition to pharyngeal muscle fate both by promoting an MRF-associated myogenic program in myoblasts and by maintaining an undifferentiated state in their sister cells through Notch-mediated lateral inhibition. The latter are stem cell-like muscle precursors that form most of the juvenile pharyngeal muscles. We discuss the implications of our findings for the development and evolution of the chordate cardiopharyngeal mesoderm.

AB - In vertebrates, pluripotent pharyngeal mesoderm progenitors produce the cardiac precursors of the second heart field as well as the branchiomeric head muscles and associated stem cells. However, the mechanisms underlying the transition from multipotent progenitors to distinct muscle precursors remain obscured by the complexity of vertebrate embryos. Using Ciona intestinalis as a simple chordate model, we show that bipotent cardiopharyngeal progenitors are primed to activate both heart and pharyngeal muscle transcriptional programs, which progressively become restricted to corresponding precursors. The transcription factor COE (Collier/OLF/EBF) orchestrates the transition to pharyngeal muscle fate both by promoting an MRF-associated myogenic program in myoblasts and by maintaining an undifferentiated state in their sister cells through Notch-mediated lateral inhibition. The latter are stem cell-like muscle precursors that form most of the juvenile pharyngeal muscles. We discuss the implications of our findings for the development and evolution of the chordate cardiopharyngeal mesoderm.

UR - http://www.scopus.com/inward/record.url?scp=84900429523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900429523&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2014.04.001

DO - 10.1016/j.devcel.2014.04.001

M3 - Article

C2 - 24794633

AN - SCOPUS:84900429523

VL - 29

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 3

ER -