Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis

Jiong Chen, Dorothea Godt, Kris Gunsalus, Istvan Kiss, Michael Goldberg, Frank A. Laski

Research output: Contribution to journalArticle

Abstract

The driving force behind cell motility is the actin cytoskeleton. Filopodia and lamellipodia are formed by the polymerization and extension of actin filaments towards the cell membrane1,2. This polymerization at the barbed end of the filament is balanced by depolymerization at the pointed end, recycling the actin in a 'treadmilling' process2,3. One protein involved in this process is cofilin/actin-depolymerizing factor (ADF), which can depolymerize actin filaments, allowing treadmilling to occur at an accelerated rate3,4. Cofilin/ADF is an actin-binding protein that is required for actin-filament disassembly, cytokinesis and the organization of muscle actin filaments4-7. There is also evidence that cofilin/ADF enhances cell motility3,8,9, although a direct requirement in vivo has not yet been shown. Here we show that Drosophila cofilin/ADF6,10, which is encoded by the twin-star (tsr) gene, promotes cell movements during ovary development and oogenesis. During larval development, cofilin/ADF is required for the cell rearrangement needed for formation of terminal filaments, stacks of somatic cells that are important for the initiation of ovarioles. It is also required for the migration of border cells during oogenesis. These results show that cofilin/ADF is an important regulator of actin-based cell motility during Drosophila development.

Original languageEnglish (US)
Pages (from-to)204-209
Number of pages6
JournalNature Cell Biology
Volume3
Issue number2
DOIs
StatePublished - 2001

Fingerprint

Destrin
Actin Depolymerizing Factors
Oogenesis
Drosophila
Cell Movement
Ovary
Actin Cytoskeleton
Actins
Pseudopodia
Polymerization
Microfilament Proteins
Cytokinesis
Recycling
Muscles

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis. / Chen, Jiong; Godt, Dorothea; Gunsalus, Kris; Kiss, Istvan; Goldberg, Michael; Laski, Frank A.

In: Nature Cell Biology, Vol. 3, No. 2, 2001, p. 204-209.

Research output: Contribution to journalArticle

Chen, Jiong ; Godt, Dorothea ; Gunsalus, Kris ; Kiss, Istvan ; Goldberg, Michael ; Laski, Frank A. / Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis. In: Nature Cell Biology. 2001 ; Vol. 3, No. 2. pp. 204-209.
@article{097f48b9ccd241f1bd820794a5aaffbe,
title = "Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis",
abstract = "The driving force behind cell motility is the actin cytoskeleton. Filopodia and lamellipodia are formed by the polymerization and extension of actin filaments towards the cell membrane1,2. This polymerization at the barbed end of the filament is balanced by depolymerization at the pointed end, recycling the actin in a 'treadmilling' process2,3. One protein involved in this process is cofilin/actin-depolymerizing factor (ADF), which can depolymerize actin filaments, allowing treadmilling to occur at an accelerated rate3,4. Cofilin/ADF is an actin-binding protein that is required for actin-filament disassembly, cytokinesis and the organization of muscle actin filaments4-7. There is also evidence that cofilin/ADF enhances cell motility3,8,9, although a direct requirement in vivo has not yet been shown. Here we show that Drosophila cofilin/ADF6,10, which is encoded by the twin-star (tsr) gene, promotes cell movements during ovary development and oogenesis. During larval development, cofilin/ADF is required for the cell rearrangement needed for formation of terminal filaments, stacks of somatic cells that are important for the initiation of ovarioles. It is also required for the migration of border cells during oogenesis. These results show that cofilin/ADF is an important regulator of actin-based cell motility during Drosophila development.",
author = "Jiong Chen and Dorothea Godt and Kris Gunsalus and Istvan Kiss and Michael Goldberg and Laski, {Frank A.}",
year = "2001",
doi = "10.1038/35055120",
language = "English (US)",
volume = "3",
pages = "204--209",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Cofilin/ADF is required for cell motility during Drosophila ovary development and oogenesis

AU - Chen, Jiong

AU - Godt, Dorothea

AU - Gunsalus, Kris

AU - Kiss, Istvan

AU - Goldberg, Michael

AU - Laski, Frank A.

PY - 2001

Y1 - 2001

N2 - The driving force behind cell motility is the actin cytoskeleton. Filopodia and lamellipodia are formed by the polymerization and extension of actin filaments towards the cell membrane1,2. This polymerization at the barbed end of the filament is balanced by depolymerization at the pointed end, recycling the actin in a 'treadmilling' process2,3. One protein involved in this process is cofilin/actin-depolymerizing factor (ADF), which can depolymerize actin filaments, allowing treadmilling to occur at an accelerated rate3,4. Cofilin/ADF is an actin-binding protein that is required for actin-filament disassembly, cytokinesis and the organization of muscle actin filaments4-7. There is also evidence that cofilin/ADF enhances cell motility3,8,9, although a direct requirement in vivo has not yet been shown. Here we show that Drosophila cofilin/ADF6,10, which is encoded by the twin-star (tsr) gene, promotes cell movements during ovary development and oogenesis. During larval development, cofilin/ADF is required for the cell rearrangement needed for formation of terminal filaments, stacks of somatic cells that are important for the initiation of ovarioles. It is also required for the migration of border cells during oogenesis. These results show that cofilin/ADF is an important regulator of actin-based cell motility during Drosophila development.

AB - The driving force behind cell motility is the actin cytoskeleton. Filopodia and lamellipodia are formed by the polymerization and extension of actin filaments towards the cell membrane1,2. This polymerization at the barbed end of the filament is balanced by depolymerization at the pointed end, recycling the actin in a 'treadmilling' process2,3. One protein involved in this process is cofilin/actin-depolymerizing factor (ADF), which can depolymerize actin filaments, allowing treadmilling to occur at an accelerated rate3,4. Cofilin/ADF is an actin-binding protein that is required for actin-filament disassembly, cytokinesis and the organization of muscle actin filaments4-7. There is also evidence that cofilin/ADF enhances cell motility3,8,9, although a direct requirement in vivo has not yet been shown. Here we show that Drosophila cofilin/ADF6,10, which is encoded by the twin-star (tsr) gene, promotes cell movements during ovary development and oogenesis. During larval development, cofilin/ADF is required for the cell rearrangement needed for formation of terminal filaments, stacks of somatic cells that are important for the initiation of ovarioles. It is also required for the migration of border cells during oogenesis. These results show that cofilin/ADF is an important regulator of actin-based cell motility during Drosophila development.

UR - http://www.scopus.com/inward/record.url?scp=0035153556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035153556&partnerID=8YFLogxK

U2 - 10.1038/35055120

DO - 10.1038/35055120

M3 - Article

VL - 3

SP - 204

EP - 209

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 2

ER -