Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase. An enzyme that preferentially regulates metabolism of very long chain ceramides

Cungui Mao, Ruijuan Xu, Zdzislaw M. Szulc, Jacek Bielawski, Kevin P. Beeker, Alicja Bielawska, Sehamuddin Galadari, Wei Hu, Lina M. Obeid

Research output: Contribution to journalArticle

Abstract

Ceramidases deacylate ceramides, important intermediates in the metabolic pathway of sphingolipids. In this study, we report the cloning and characterization of a novel mouse alkaline ceramidase (maCER1) with a highly restricted substrate specificity. maCER1 consists of 287 amino acids, and it has a 28 and 32% identity to the Saccharomyces alkaline ceramidases (YPC1p and YDC1p) and the human alkaline phytoceramidase, respectively. Reverse transcriptase-PCR analysis demonstrated that maCER1 was predominantly expressed in skin. maCER1 was localized to the endoplasmic reticulum as revealed by immunocytochemistry. In vitro biochemical characterization determined that maCER1 hydrolyzed D-erythro-ceramide exclusively but not D-erythro-dihydroceramide or D-ribo-phytoceramide. Similar to other alkaline ceramidases, maCER1 had an alkaline pH optimum of 8.0, and it was activated by Ca2+ but inhibited by Zn2+, Cu2+, and Mn 2+. maCER1 was also inhibited by sphingosine, one of its products. Metabolic labeling studies showed that overexpression of maCER1 caused a decrease in the incorporation of radiolabeled dihydrosphingosine into ceramide and complex sphingolipids but led to a concomitant increase in sphingosine-1-P (S1P) in HeLa cells. Mass measurement showed that overexpression of maCER1 selectively lowered the cellular levels of D-erythro-C24:1-ceramide, but not other ceramide species and caused an increase in the levels of S1P. Taken together, these data suggest that maCER1 is a novel alkaline ceramidase with a stringent substrate specificity and that maCER1 is selectively expressed in skin and may have a role in regulating the levels of bioactive lipids ceramide and S1P, as well as complex sphingolipids.

Original languageEnglish (US)
Pages (from-to)31184-31191
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number33
DOIs
StatePublished - Aug 15 2003

Fingerprint

Alkaline Ceramidase
Ceramides
Cloning
Metabolism
Endoplasmic Reticulum
Organism Cloning
Sphingosine
Sphingolipids
Enzymes
Substrate Specificity
Skin
Ceramidases
Saccharomyces
RNA-Directed DNA Polymerase
Substrates
Metabolic Networks and Pathways
Reverse Transcriptase Polymerase Chain Reaction
HeLa Cells
Labeling
Immunohistochemistry

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase. An enzyme that preferentially regulates metabolism of very long chain ceramides. / Mao, Cungui; Xu, Ruijuan; Szulc, Zdzislaw M.; Bielawski, Jacek; Beeker, Kevin P.; Bielawska, Alicja; Galadari, Sehamuddin; Hu, Wei; Obeid, Lina M.

In: Journal of Biological Chemistry, Vol. 278, No. 33, 15.08.2003, p. 31184-31191.

Research output: Contribution to journalArticle

Mao, Cungui ; Xu, Ruijuan ; Szulc, Zdzislaw M. ; Bielawski, Jacek ; Beeker, Kevin P. ; Bielawska, Alicja ; Galadari, Sehamuddin ; Hu, Wei ; Obeid, Lina M. / Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase. An enzyme that preferentially regulates metabolism of very long chain ceramides. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 33. pp. 31184-31191.
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abstract = "Ceramidases deacylate ceramides, important intermediates in the metabolic pathway of sphingolipids. In this study, we report the cloning and characterization of a novel mouse alkaline ceramidase (maCER1) with a highly restricted substrate specificity. maCER1 consists of 287 amino acids, and it has a 28 and 32{\%} identity to the Saccharomyces alkaline ceramidases (YPC1p and YDC1p) and the human alkaline phytoceramidase, respectively. Reverse transcriptase-PCR analysis demonstrated that maCER1 was predominantly expressed in skin. maCER1 was localized to the endoplasmic reticulum as revealed by immunocytochemistry. In vitro biochemical characterization determined that maCER1 hydrolyzed D-erythro-ceramide exclusively but not D-erythro-dihydroceramide or D-ribo-phytoceramide. Similar to other alkaline ceramidases, maCER1 had an alkaline pH optimum of 8.0, and it was activated by Ca2+ but inhibited by Zn2+, Cu2+, and Mn 2+. maCER1 was also inhibited by sphingosine, one of its products. Metabolic labeling studies showed that overexpression of maCER1 caused a decrease in the incorporation of radiolabeled dihydrosphingosine into ceramide and complex sphingolipids but led to a concomitant increase in sphingosine-1-P (S1P) in HeLa cells. Mass measurement showed that overexpression of maCER1 selectively lowered the cellular levels of D-erythro-C24:1-ceramide, but not other ceramide species and caused an increase in the levels of S1P. Taken together, these data suggest that maCER1 is a novel alkaline ceramidase with a stringent substrate specificity and that maCER1 is selectively expressed in skin and may have a role in regulating the levels of bioactive lipids ceramide and S1P, as well as complex sphingolipids.",
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AU - Xu, Ruijuan

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AU - Bielawski, Jacek

AU - Beeker, Kevin P.

AU - Bielawska, Alicja

AU - Galadari, Sehamuddin

AU - Hu, Wei

AU - Obeid, Lina M.

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AB - Ceramidases deacylate ceramides, important intermediates in the metabolic pathway of sphingolipids. In this study, we report the cloning and characterization of a novel mouse alkaline ceramidase (maCER1) with a highly restricted substrate specificity. maCER1 consists of 287 amino acids, and it has a 28 and 32% identity to the Saccharomyces alkaline ceramidases (YPC1p and YDC1p) and the human alkaline phytoceramidase, respectively. Reverse transcriptase-PCR analysis demonstrated that maCER1 was predominantly expressed in skin. maCER1 was localized to the endoplasmic reticulum as revealed by immunocytochemistry. In vitro biochemical characterization determined that maCER1 hydrolyzed D-erythro-ceramide exclusively but not D-erythro-dihydroceramide or D-ribo-phytoceramide. Similar to other alkaline ceramidases, maCER1 had an alkaline pH optimum of 8.0, and it was activated by Ca2+ but inhibited by Zn2+, Cu2+, and Mn 2+. maCER1 was also inhibited by sphingosine, one of its products. Metabolic labeling studies showed that overexpression of maCER1 caused a decrease in the incorporation of radiolabeled dihydrosphingosine into ceramide and complex sphingolipids but led to a concomitant increase in sphingosine-1-P (S1P) in HeLa cells. Mass measurement showed that overexpression of maCER1 selectively lowered the cellular levels of D-erythro-C24:1-ceramide, but not other ceramide species and caused an increase in the levels of S1P. Taken together, these data suggest that maCER1 is a novel alkaline ceramidase with a stringent substrate specificity and that maCER1 is selectively expressed in skin and may have a role in regulating the levels of bioactive lipids ceramide and S1P, as well as complex sphingolipids.

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