Cloning and characterization of a developmentally regulated sea urchin cDNA encoding glutamine synthetase

Laura Fucci, Annamaria Piscopo, Francesco Aniello, Margherita Branno, Anna Di Gregorio, Raffaele Calogero, Giuseppe Geraci

Research output: Contribution to journalArticle

Abstract

A 2935-bp cDNA clone encoding glutamine synthetase (GS) was isolated from a cDNA library prepared from four-blastomere Paracentrotus lividus sea urchin embryos. The sequence consists of a 75-bp 5′ untranslated region (5′-UTR) followed by a 1095-bp coding region corresponding to a 365-amino-acid (aa) protein, a 1747-bp 3′-UTR and a terminal 18-bp poly(A) tail. The encoded protein shows about 66% identical residues, as compared with human and lobster class-II GS. The sequence contains the Mn2+-binding aa and the highly conserved aa regions observed in other GS. Northern blot analyses show that the GS mRNA is present in the sea urchin egg and is developmentally regulated in the embryo.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalGene
Volume152
Issue number2
DOIs
StatePublished - Jan 23 1995

Fingerprint

Glutamate-Ammonia Ligase
Sea Urchins
Organism Cloning
Complementary DNA
Amino Acids
Embryonic Structures
Paracentrotus
Blastomeres
Messenger RNA
5' Untranslated Regions
3' Untranslated Regions
Gene Library
Northern Blotting
Ovum
Proteins
Clone Cells

Keywords

  • comparison with human and lobster GS
  • embryo
  • mRNA control
  • Paracentrotus lividus
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning and characterization of a developmentally regulated sea urchin cDNA encoding glutamine synthetase. / Fucci, Laura; Piscopo, Annamaria; Aniello, Francesco; Branno, Margherita; Di Gregorio, Anna; Calogero, Raffaele; Geraci, Giuseppe.

In: Gene, Vol. 152, No. 2, 23.01.1995, p. 205-208.

Research output: Contribution to journalArticle

Fucci, Laura ; Piscopo, Annamaria ; Aniello, Francesco ; Branno, Margherita ; Di Gregorio, Anna ; Calogero, Raffaele ; Geraci, Giuseppe. / Cloning and characterization of a developmentally regulated sea urchin cDNA encoding glutamine synthetase. In: Gene. 1995 ; Vol. 152, No. 2. pp. 205-208.
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AU - Calogero, Raffaele

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