Circadian rhythms in neuronal activity propagate through output circuits

Matthieu Cavey, Ben Collins, Claire Bertet, Justin Blau

Research output: Contribution to journalArticle

Abstract

Twenty-four hour rhythms in behavior are organized by a network of circadian pacemaker neurons. Rhythmic activity in this network is generated by intrinsic rhythms in clock neuron physiology and communication between clock neurons. However, it is poorly understood how the activity of a small number of pacemaker neurons is translated into rhythmic behavior of the whole animal. To understand this, we screened for signals that could identify circadian output circuits in Drosophila melanogaster. We found that leucokinin neuropeptide (LK) and its receptor (LK-R) were required for normal behavioral rhythms. This LK/LK-R circuit connects pacemaker neurons to brain areas that regulate locomotor activity and sleep. Our experiments revealed that pacemaker neurons impose rhythmic activity and excitability on LK- and LK-R-expressing neurons. We also found pacemaker neuron–dependent activity rhythms in a second circadian output pathway controlled by DH44 neuropeptide–expressing neurons. We conclude that rhythmic clock neuron activity propagates to multiple downstream circuits to orchestrate behavioral rhythms.

Original languageEnglish (US)
JournalNature Neuroscience
DOIs
StateAccepted/In press - Feb 29 2016

Fingerprint

Circadian Rhythm
Neurons
Neuropeptides
Neuropeptide Receptors
Animal Behavior
Locomotion
Drosophila melanogaster
Sleep
Communication
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Circadian rhythms in neuronal activity propagate through output circuits. / Cavey, Matthieu; Collins, Ben; Bertet, Claire; Blau, Justin.

In: Nature Neuroscience, 29.02.2016.

Research output: Contribution to journalArticle

@article{dcbd239e058b4eb9934d05c957c79200,
title = "Circadian rhythms in neuronal activity propagate through output circuits",
abstract = "Twenty-four hour rhythms in behavior are organized by a network of circadian pacemaker neurons. Rhythmic activity in this network is generated by intrinsic rhythms in clock neuron physiology and communication between clock neurons. However, it is poorly understood how the activity of a small number of pacemaker neurons is translated into rhythmic behavior of the whole animal. To understand this, we screened for signals that could identify circadian output circuits in Drosophila melanogaster. We found that leucokinin neuropeptide (LK) and its receptor (LK-R) were required for normal behavioral rhythms. This LK/LK-R circuit connects pacemaker neurons to brain areas that regulate locomotor activity and sleep. Our experiments revealed that pacemaker neurons impose rhythmic activity and excitability on LK- and LK-R-expressing neurons. We also found pacemaker neuron–dependent activity rhythms in a second circadian output pathway controlled by DH44 neuropeptide–expressing neurons. We conclude that rhythmic clock neuron activity propagates to multiple downstream circuits to orchestrate behavioral rhythms.",
author = "Matthieu Cavey and Ben Collins and Claire Bertet and Justin Blau",
year = "2016",
month = "2",
day = "29",
doi = "10.1038/nn.4263",
language = "English (US)",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Circadian rhythms in neuronal activity propagate through output circuits

AU - Cavey, Matthieu

AU - Collins, Ben

AU - Bertet, Claire

AU - Blau, Justin

PY - 2016/2/29

Y1 - 2016/2/29

N2 - Twenty-four hour rhythms in behavior are organized by a network of circadian pacemaker neurons. Rhythmic activity in this network is generated by intrinsic rhythms in clock neuron physiology and communication between clock neurons. However, it is poorly understood how the activity of a small number of pacemaker neurons is translated into rhythmic behavior of the whole animal. To understand this, we screened for signals that could identify circadian output circuits in Drosophila melanogaster. We found that leucokinin neuropeptide (LK) and its receptor (LK-R) were required for normal behavioral rhythms. This LK/LK-R circuit connects pacemaker neurons to brain areas that regulate locomotor activity and sleep. Our experiments revealed that pacemaker neurons impose rhythmic activity and excitability on LK- and LK-R-expressing neurons. We also found pacemaker neuron–dependent activity rhythms in a second circadian output pathway controlled by DH44 neuropeptide–expressing neurons. We conclude that rhythmic clock neuron activity propagates to multiple downstream circuits to orchestrate behavioral rhythms.

AB - Twenty-four hour rhythms in behavior are organized by a network of circadian pacemaker neurons. Rhythmic activity in this network is generated by intrinsic rhythms in clock neuron physiology and communication between clock neurons. However, it is poorly understood how the activity of a small number of pacemaker neurons is translated into rhythmic behavior of the whole animal. To understand this, we screened for signals that could identify circadian output circuits in Drosophila melanogaster. We found that leucokinin neuropeptide (LK) and its receptor (LK-R) were required for normal behavioral rhythms. This LK/LK-R circuit connects pacemaker neurons to brain areas that regulate locomotor activity and sleep. Our experiments revealed that pacemaker neurons impose rhythmic activity and excitability on LK- and LK-R-expressing neurons. We also found pacemaker neuron–dependent activity rhythms in a second circadian output pathway controlled by DH44 neuropeptide–expressing neurons. We conclude that rhythmic clock neuron activity propagates to multiple downstream circuits to orchestrate behavioral rhythms.

UR - http://www.scopus.com/inward/record.url?scp=84959200804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959200804&partnerID=8YFLogxK

U2 - 10.1038/nn.4263

DO - 10.1038/nn.4263

M3 - Article

JO - Nature Neuroscience

JF - Nature Neuroscience

SN - 1097-6256

ER -