Cinnamate-based DNA photolithography

Lang Feng, Joy Romulus, Minfeng Li, Ruojie Sha, John Royer, Kun Ta Wu, Qin Xu, Nadrian Seeman, Marcus Weck, Paul Chaikin

Research output: Contribution to journalArticle

Abstract

As demonstrated by means of DNA nanoconstructs, as well as DNA functionalization of nanoparticles and micrometre-scale colloids, complex self-assembly processes require components to associate with particular partners in a programmable fashion. In many cases the reversibility of the interactions between complementary DNA sequences is an advantage. However, permanently bonding some or all of the complementary pairs may allow for flexibility in design and construction. Here, we show that the substitution of a cinnamate group for a pair of complementary bases provides an efficient, addressable, ultraviolet light-based method to bond complementary DNA covalently. To show the potential of this approach, we wrote micrometre-scale patterns on a surface using ultraviolet light and demonstrated the reversible attachment of conjugated DNA and DNA-coated colloids. Our strategy enables both functional DNA photolithography and multistep, specific binding in self-assembly processes.

Original languageEnglish (US)
Pages (from-to)747-753
Number of pages7
JournalNature Materials
Volume12
Issue number8
DOIs
StatePublished - Aug 2013

Fingerprint

Cinnamates
Photolithography
photolithography
DNA
deoxyribonucleic acid
complementary DNA
Colloids
Ultraviolet Rays
ultraviolet radiation
Self assembly
colloids
self assembly
micrometers
Complementary DNA
DNA sequences
Base Pairing
Nanoparticles
attachment
flexibility
Substitution reactions

ASJC Scopus subject areas

  • Mechanical Engineering
  • Mechanics of Materials
  • Condensed Matter Physics
  • Materials Science(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Feng, L., Romulus, J., Li, M., Sha, R., Royer, J., Wu, K. T., ... Chaikin, P. (2013). Cinnamate-based DNA photolithography. Nature Materials, 12(8), 747-753. https://doi.org/10.1038/nmat3645

Cinnamate-based DNA photolithography. / Feng, Lang; Romulus, Joy; Li, Minfeng; Sha, Ruojie; Royer, John; Wu, Kun Ta; Xu, Qin; Seeman, Nadrian; Weck, Marcus; Chaikin, Paul.

In: Nature Materials, Vol. 12, No. 8, 08.2013, p. 747-753.

Research output: Contribution to journalArticle

Feng, L, Romulus, J, Li, M, Sha, R, Royer, J, Wu, KT, Xu, Q, Seeman, N, Weck, M & Chaikin, P 2013, 'Cinnamate-based DNA photolithography', Nature Materials, vol. 12, no. 8, pp. 747-753. https://doi.org/10.1038/nmat3645
Feng L, Romulus J, Li M, Sha R, Royer J, Wu KT et al. Cinnamate-based DNA photolithography. Nature Materials. 2013 Aug;12(8):747-753. https://doi.org/10.1038/nmat3645
Feng, Lang ; Romulus, Joy ; Li, Minfeng ; Sha, Ruojie ; Royer, John ; Wu, Kun Ta ; Xu, Qin ; Seeman, Nadrian ; Weck, Marcus ; Chaikin, Paul. / Cinnamate-based DNA photolithography. In: Nature Materials. 2013 ; Vol. 12, No. 8. pp. 747-753.
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