Cihox5, a new Ciona intestinalis Hox-related gene, is involved in regionalization of the spinal cord

Michela Gionti, Filomena Ristoratore, Anna Di Gregorio, Francesco Aniello, Margherita Branno, Roberto Di Lauro

Research output: Contribution to journalArticle

Abstract

In this paper we report the cloning, sequence and expression analysis of a new Ciona intestinalis hox gene. On the basis of sequence comparison with mammalian and Amphioxus homologues, we called this gene Cihox5. Northern blot analysis reveals a single transcript of about 1.3 kb in length, that is present from neurula until larva stage. Whole-mount in situ hybridization shows restricted expression of this gene in putative blood cells precursors and in a regional domain of the spinal chord. Expression in the spinal cord is attributed to ependymal cells. This result implies a role for this gene in primitive regionalization of spinal cord along the anteroposterior axis in the absence of neuronal bodies.

Original languageEnglish (US)
Pages (from-to)515-523
Number of pages9
JournalDevelopment Genes and Evolution
Volume207
Issue number8
DOIs
StatePublished - 1998

Fingerprint

Ciona intestinalis
Homeobox Genes
regionalization
spinal cord
Spinal Cord
Lancelets
gene
Northern Blotting
Genes
In Situ Hybridization
Larva
Sequence Analysis
Organism Cloning
Blood Cells
genes
blood cells
Gene Expression
Northern blotting
in situ hybridization
molecular cloning

Keywords

  • Ascidians
  • CNS regionalization
  • Hox
  • Neural tube

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Cell Biology
  • Developmental Biology
  • Genetics

Cite this

Cihox5, a new Ciona intestinalis Hox-related gene, is involved in regionalization of the spinal cord. / Gionti, Michela; Ristoratore, Filomena; Di Gregorio, Anna; Aniello, Francesco; Branno, Margherita; Di Lauro, Roberto.

In: Development Genes and Evolution, Vol. 207, No. 8, 1998, p. 515-523.

Research output: Contribution to journalArticle

Gionti, Michela ; Ristoratore, Filomena ; Di Gregorio, Anna ; Aniello, Francesco ; Branno, Margherita ; Di Lauro, Roberto. / Cihox5, a new Ciona intestinalis Hox-related gene, is involved in regionalization of the spinal cord. In: Development Genes and Evolution. 1998 ; Vol. 207, No. 8. pp. 515-523.
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