Cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in peripheral mononuclear cells

Regina M. Santella, Ricardo A. Grinberg-Funes, Tie Lan Young, Chris Dickey, Vishwa Nath Singh, Lian Wen Wang, Frederica P. Perera

Research output: Contribution to journalArticle

Abstract

Studies on cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in blood have produced conflicting results. To determine whether a subset of specific white blood cells is a useful marker for monitoring exposure to cigarette smoke, blood was obtained from 63 heavy smokers and 27 non-smokers. Adduct levels were determined by competitive enzyme-linked immunosorbent assay with a polyclonal antiserum recognizing benzo[a]pyrene and structurally related diolepoxide - DNA adducts. Analysis of the lymphocyte plus monocyte fraction from smokers indicated 70% had detectable adducts with a mean of 4.38 ± 4.29 adducts/108 nucleotides, while in non-smokers the corresponding values were 22% and 1.35 ± 0.78/108 (P <0.001). Plasma cotinine levels differed significantly in smokers (286 ± 90 μg/1) compared to non-smokers (4.4 ± 3.3 μg/1) (P <0.001). However, cotinine was not correlated with self-reported smoking history in these heavy smokers. Nor were DNA adducts in smokers correlated with cigarettes per day, pack-years and plasma cotinine, indicating large interindividual variation in DNA adduct formation. These data demonstrate lymphocytes plus monocytes from smokers have elevated levels of polycyclic aromatic hydrocarbon diolepoxide - DNA adduct levels compared to non-smokers.

Original languageEnglish (US)
Pages (from-to)2041-2045
Number of pages5
JournalCarcinogenesis
Volume13
Issue number11
StatePublished - Nov 1992

Fingerprint

Cotinine
DNA Adducts
Smoking
Hydrocarbons
Polycyclic aromatic hydrocarbons
Monocytes
Blood
Lymphocytes
Tobacco Products
Cell
Plasma
Enzyme-linked Immunosorbent Assay
Benzo(a)pyrene
Smoke
Immune Sera
Leukocytes
Plasmas
Nucleotides
History
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

Cite this

Santella, R. M., Grinberg-Funes, R. A., Young, T. L., Dickey, C., Singh, V. N., Wang, L. W., & Perera, F. P. (1992). Cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in peripheral mononuclear cells. Carcinogenesis, 13(11), 2041-2045.

Cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in peripheral mononuclear cells. / Santella, Regina M.; Grinberg-Funes, Ricardo A.; Young, Tie Lan; Dickey, Chris; Singh, Vishwa Nath; Wang, Lian Wen; Perera, Frederica P.

In: Carcinogenesis, Vol. 13, No. 11, 11.1992, p. 2041-2045.

Research output: Contribution to journalArticle

Santella, RM, Grinberg-Funes, RA, Young, TL, Dickey, C, Singh, VN, Wang, LW & Perera, FP 1992, 'Cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in peripheral mononuclear cells', Carcinogenesis, vol. 13, no. 11, pp. 2041-2045.
Santella RM, Grinberg-Funes RA, Young TL, Dickey C, Singh VN, Wang LW et al. Cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in peripheral mononuclear cells. Carcinogenesis. 1992 Nov;13(11):2041-2045.
Santella, Regina M. ; Grinberg-Funes, Ricardo A. ; Young, Tie Lan ; Dickey, Chris ; Singh, Vishwa Nath ; Wang, Lian Wen ; Perera, Frederica P. / Cigarette smoking related polycyclic aromatic hydrocarbon - DNA adducts in peripheral mononuclear cells. In: Carcinogenesis. 1992 ; Vol. 13, No. 11. pp. 2041-2045.
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