Chymotrypsin C (caldecrin) is associated with enamel development

Rodrigo Lacruz, C. E. Smith, S. M. Smith, P. Hu, P. Bringas, M. Sahin-Tóth, J. Moradian-Oldak, M. L. Paine

Research output: Contribution to journalArticle

Abstract

Two main proteases cleave enamel extracellular matrix proteins during amelogenesis. Matrix metalloprotease-20 (Mmp20) is the predominant enzyme expressed during the secretory stage, while kallikrein-related peptidase-4 (Klk4) is predominantly expressed during maturation. Mutations to both Mmp20 and Klk4 result in abnormal enamel phenotypes. During a recent whole-genome microarray analysis of rat incisor enamel organ cells derived from the secretory and maturation stages of amelogenesis, the serine protease chymotrypsin C (caldecrin, Ctrc) was identified as significantly up-regulated (> 11-fold) during enamel maturation. Prior reports indicate that Ctrc expression is pancreas-specific, albeit low levels were also noted in brain. We here report on the expression of Ctrc in the enamel organ. Quantitative PCR (qPCR) and Western blot analysis were used to confirm the expression of Ctrc in the developing enamel organ. The expression profile of Ctrc is similar to that of Klk4, increasing markedly during the maturation stage relative to the secretory stage, although levels of Ctrc mRNA are lower than for Klk4. The discovery of a new serine protease possibly involved in enamel development has important implications for our understanding of the factors that regulate enamel biomineralization.

Original languageEnglish (US)
Pages (from-to)1228-1233
Number of pages6
JournalJournal of Dental Research
Volume90
Issue number10
DOIs
StatePublished - Oct 2011

Fingerprint

Dental Enamel
Kallikreins
Enamel Organ
Peptide Hydrolases
Amelogenesis
Metalloproteases
Serine Proteases
Extracellular Matrix Proteins
Incisor
Microarray Analysis
Pancreas
Western Blotting
chymotrypsin C
caldecrin
Genome
Phenotype
Polymerase Chain Reaction
Messenger RNA
Mutation
Brain

Keywords

  • amelogenesis
  • biomineralization
  • caldecrin
  • chymotrypsin C
  • CTRC
  • enamel
  • serine proteases

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Lacruz, R., Smith, C. E., Smith, S. M., Hu, P., Bringas, P., Sahin-Tóth, M., ... Paine, M. L. (2011). Chymotrypsin C (caldecrin) is associated with enamel development. Journal of Dental Research, 90(10), 1228-1233. https://doi.org/10.1177/0022034511418231

Chymotrypsin C (caldecrin) is associated with enamel development. / Lacruz, Rodrigo; Smith, C. E.; Smith, S. M.; Hu, P.; Bringas, P.; Sahin-Tóth, M.; Moradian-Oldak, J.; Paine, M. L.

In: Journal of Dental Research, Vol. 90, No. 10, 10.2011, p. 1228-1233.

Research output: Contribution to journalArticle

Lacruz, R, Smith, CE, Smith, SM, Hu, P, Bringas, P, Sahin-Tóth, M, Moradian-Oldak, J & Paine, ML 2011, 'Chymotrypsin C (caldecrin) is associated with enamel development', Journal of Dental Research, vol. 90, no. 10, pp. 1228-1233. https://doi.org/10.1177/0022034511418231
Lacruz, Rodrigo ; Smith, C. E. ; Smith, S. M. ; Hu, P. ; Bringas, P. ; Sahin-Tóth, M. ; Moradian-Oldak, J. ; Paine, M. L. / Chymotrypsin C (caldecrin) is associated with enamel development. In: Journal of Dental Research. 2011 ; Vol. 90, No. 10. pp. 1228-1233.
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